Fabrication of a magnetic metal-organic framework/covalent organic framework composite for simultaneous magnetic solid-phase extraction of seventeen trace quinolones residues in meats

• Published in: Journal of Chromatography A 2023-10, Vol.1709, p.464403, Article 464403
• Main Authors: Lin, Shumin, Liang, Bolong, Zhao, Zhe, Li, Zhenqiu, Deng, Kai, He, Hongbin, Liang, Shu-xuan
• Format: Article
• Language: English
• Subjects: Covalent organic frameworks; HPLC-Q-orbitrap mass spectrometry; Magnetic solid-phase extraction; Metal-organic frameworks; Quinolones residues
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2023.464403

•A selective adsorbent of Fe3O4@NH2−MIL-125@TpPa-SO3H for QNs was first fabricated.•Fe3O4@NH2−MIL-125@TpPa-SO3H was developed as a novel adsorbent for MSPE.•A MSPE-HPLC-HRMS method was established for the determination of 17 QNs in meats.•The proposed method provided satisfactory performance in linearity and sensitivity.•Adsorption mechanism of Fe3O4@NH2−MIL-125@TpPa-SO3H was discussed in detail. Effective capture of quinolones (QNs) in animal-derived food is a vital procedure for food safety monitoring. However, the lack of adsorption specificity and difficult to recycle in complex substrate conditions have been major problems for most of the adsorbents. In this work, a magnetic Fe3O4/MOF/COF composite (named Fe3O4@NH2−MIL-125@TpPa-SO3H) was successfully synthesized with good magnetic responsiveness and conspicuous affinity towards QNs. The Fe3O4/MOF/COF composite was used as a magnetic solid-phase extraction (MSPE) adsorbent for pretreatment and determination of QNs in meat samples. Under optimal MSPE conditions in combination with high performance liquid chromatography-quadrupole orbitrap high resolution mass spectrometer (HPLC-Q-Orbitrap HRMS), the proposed method had good linearity (R2 ≥ 0.9978) from 0.01 to 100ng g−1, low limits of detection (0.0016 to 0.0940ng g−1), good precision with relative standard deviations lower than 5.8%. This method was effectively applied to the detection of 17 QNs in the spiked pork, chicken and beef samples with satisfactory recoveries from 83.9 to 106.2%. The separation selectivity mainly due to the π–π interaction, hydrogen bonding, and electrostatic attraction between QNs and the sulfonic acid and amino functional groups of the composite. After verification, the stability and reusability of the composite meet the requirements of complex matrix sample pretreatment. The developed MSPE method based on the magnetic Fe3O4/MOF/COF composite provided an ideal sample pretreatment alternative for determining trace QNs in complex matrixes with selectivity, simplicity, rapidity, and efficiency.