On-line coupling of hollow-fiber flow field-flow fractionation and depolarized multi-angle static light scattering (HF5/D-MALS). Proof of principle

•On-line coupling of HF5 to depolarized MALS is demonstrated.•HF5/D-MALS applied to study of a Teflon latex.•Depolarization ratio of latex measured continuously across fractogram. Introduced here is the on-line coupling of hollow-fiber flow field-flow fractionation (HF5) to depolarized multi-angle s...

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Bibliographic Details
Published in:Journal of Chromatography A 2024-08, Vol.1730, p.465115, Article 465115
Main Author: Striegel, André M.
Format: Article
Language:English
Subjects:
Depolarization ratio
Depolarized multi-angle static light scattering
Hollow-fiber flow field-flow fractionation
On-line coupling
Teflon latex
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Summary:•On-line coupling of HF5 to depolarized MALS is demonstrated.•HF5/D-MALS applied to study of a Teflon latex.•Depolarization ratio of latex measured continuously across fractogram. Introduced here is the on-line coupling of hollow-fiber flow field-flow fractionation (HF5) to depolarized multi-angle static light scattering (D-MALS). HF5 is a size-based separation alternative to size-exclusion and hydrodynamic chromatography and asymmetric flow field-flow fractionation. HF5 can separate larger sizes than its chromatographic counterparts and provides several advantages over its fractionation counterpart, including reduced sample consumption and greater ease of operation. D-MALS is a variant of MALS in which the depolarized scattering from the analyte solution is measured at a variety of angles simultaneously. Measurements of depolarized scattering have previously been employed in studying the optical properties of solutions or suspensions, to determine the length of rod-like analytes, and to gain increased accuracy in the determination of analyte molar mass. The coupling HF5/D-MALS allows for the depolarization ratio of a solution or suspension to be measured continuously across the fractogram. This is demonstrated here for a Teflon latex the size range of which extends beyond that accessible to commercial size-exclusion columns. The results presented provide the first reported on-line HF5/D-MALS coupling, showing the feasibility of the technique as well as its realized potential for providing continuous depolarization measurements, inter alia.
ISSN:0021-9673
DOI:10.1016/j.chroma.2024.465115