From grape to wine: A thorough compound specific isotopic, enantiomeric and quali-quantitative investigation by means of gas chromatographic analysis

• Published in: Journal of Chromatography A 2024-08, Vol.1730, p.465149, Article 465149
• Main Authors: Cucinotta, Lorenzo, Cannizzaro, Francesca, Paolini, Mauro, Roncone, Alberto, Camin, Federica, Bontempo, Luana, Larcher, Roberto, Sciarrone, Danilo, Mondello, Luigi
• Format: Article
• Language: English
• Subjects: Enantioselective gas chromatography; Isotopic ratio mass spectrometry; Multidimensional gas chromatography; Terpenoids; Wine
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2024.465149

•Comprehensive characterization of Moscato giallo grape and wines.•Multidimensional gas chromatography to overcome issues associated to co-elutions.•Evaluation of the enantiomeric distribution of the main chiral terpenes along the winemaking process.•The isotopic distribution of the enantiomeric forms in grape and wine was determined for the first time in literature. In this study, multiple analytical approaches, including simultaneous enantiomeric and isotopic analysis, were employed to thoroughly investigate the volatile fraction in Moscato giallo grape berries and wines. For the qualitative and quantitative profiling, a fast GC-QqQ/MS approach was successfully utilized. However, prior to isotopic analysis, the extracts underwent an additional concentration step, necessitating an assessment of isotopic fractionation during the concentration process. Once the absence of carbon isotopic fractionation was confirmed, this research aimed to develop a suitable gas chromatographic method for the simultaneous detection of both enantiomeric and isotopic ratios of target monoterpenoids in Moscato giallo samples. To address the limitations associated with a one-dimensional approach, multidimensional gas chromatography was employed to enhance separation before IRMS and qMS detections. Utilizing a Deans switch transfer device, the coupling of an apolar column in the first dimension and a chiral cyclodextrin-based stationary phase in the second dimension proved effective for this purpose. The data obtained from the analysis of Moscato giallo samples allowed for the assessment of natural isotopic and enantiomeric distributions in grapes and wines for the first time in the literature. Significant enantiomeric excesses were observed for the target terpenoids investigated. Regarding isotopic distribution, a consistent trend was observed for all detected target terpenols, including the linalool enantiomers. To date, this study represents the first investigation of simultaneous δ13C and chiral investigation of the main terpenoids in oenological products in the literature.