Fabrication of norepinephrine and ε-polylysine engineered magnetic nanocomposites tailored for phosphopeptides analysis

•A novel magnetic nanocomposite, Fe3O4@NE@PL, was synthesized for phosphopeptides enrichment.•The material demonstrated high sensitivity and selectivity for phosphopeptide enrichment.•Fe3O4@NE@PL was successfully applied to enrich phosphopeptides from complex real biological samples such as human se...

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Bibliographic Details
Published in:Journal of Chromatography A 2025-01, Vol.1739, p.465539, Article 465539
Main Authors: Zhao, Yaming, Zhang, Jinfeng, Yang, JunWei, Ma, Jiutong, Zhang, Haipeng, Jia, Qiong
Format: Article
Language:English
Subjects:
adsorbents
blood serum
chromatography
humans
hydrogen
hydrophilicity
Lewis acids
Magnetic nanocomposite
magnetism
mass spectrometry
nanocomposites
Norepinephrine
Phosphopeptide enrichment
phosphopeptides
post-translational modification
protein phosphorylation
proteomics
saliva
ε-polylysine
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Summary:•A novel magnetic nanocomposite, Fe3O4@NE@PL, was synthesized for phosphopeptides enrichment.•The material demonstrated high sensitivity and selectivity for phosphopeptide enrichment.•Fe3O4@NE@PL was successfully applied to enrich phosphopeptides from complex real biological samples such as human serum and saliva.•The material showed favorable stability and reusability, achieving up to 4 recycles with excellent performance. Protein phosphorylation is a highly prevalent post-translational modification that holds a vital position in numerous physiological processes. Prior to mass spectrometry detection, the enrichment of phosphopeptides is critically significant due to their susceptibility to interference from abundant non-phosphopeptides. In this study, the magnetic nanocomposite (Fe3O4@NE@PL) was successfully synthesized and characterized. Fe3O4@NE@PL exhibited strong hydrophilicity, electrophilicity and intermolecular interactions through hydrogen bonds, enabling it to effectively enrich phosphopeptides with excellent sensitivity (0.4 fmol β-casein) and selectivity (β-casein:BSA=1:1000). In addition, Fe3O4@NE@PL was successfully applied to enrich phosphopeptides from complex real biological samples such as human serum and saliva, achieving up to 4 recycles with favorable stability and reusability. This study demonstrates that Fe3O4@NE@PL is a promising adsorbent for phosphopeptides enrichment in proteomics research, providing new ideas for the construction of magnetic enrichment materials.
ISSN:0021-9673
DOI:10.1016/j.chroma.2024.465539