Histidine modified montmorillonite: Laccase immobilization and application to flow injection analysis of phenols

The preparation, characterization and application of histidine (His) modified montmorillonite (His-Mt) in electrochemical biosensor fabrication were reported. His was used as a modifier to enhance immobilization capability of Mt for the biomolecules. Mt modifications were followed by X-ray diffracti...

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Bibliographic Details
Published in:Applied clay science 2013-12, Vol.86, p.64-69
Main Authors: Songurtekin, Didem, Yalcinkaya, Esra Evrim, Ag, Didem, Seleci, Muharrem, Demirkol, Dilek Odaci, Timur, Suna
Format: Article
Language:English
Subjects:
biosensing of phenolic compounds
Earth sciences
Earth, ocean, space
Exact sciences and technology
Laccase
Mineralogy
Modification of clay mineral
Montmorillonite
Silicates
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Summary:The preparation, characterization and application of histidine (His) modified montmorillonite (His-Mt) in electrochemical biosensor fabrication were reported. His was used as a modifier to enhance immobilization capability of Mt for the biomolecules. Mt modifications were followed by X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetry analysis. After a successful immobilization of Laccase enzyme on the His-Mt matrix, covered on a glassy carbon electrode, application in catechol biosensing was investigated in detail. Optimization, analytical characteristics, repeatability, and operational stability studies were evaluated using both batch and flow injections analysis configurations. [Display omitted] •Na+-montmorillonite was intercalated with Histidine amino acid.•A phenol biosensor consist of immobilized laccase using histidine-modified montmorillonite•Optimized biosensor for best response and electrochemical behavior in addition to analytical characteristics
ISSN:0169-1317
1872-9053
DOI:10.1016/j.clay.2013.10.014