A simple and inexpensive automated technique for measurement of serum nitrite/nitrate

We described an automated technique for measurement of serum nitrite/nitrate (NO x ) using the Cobas Mira clinical chemistry analyzer. NO x was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed. The auto...

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Bibliographic Details
Published in:Clinical biochemistry 2011-03, Vol.44 (4), p.348-350
Main Authors: Tatsch, Etiane, Bochi, Guilherme Vargas, Pereira, Renata da Silva, Kober, Helena, Agertt, Vanessa Albertina, Anraku de Campos, Marli Matiko, Gomes, Patrícia, Duarte, Marta Maria Medeiros Frescura, Moresco, Rafael Noal
Format: Article
Language:English
Subjects:
Automation, Laboratory
Biological and medical sciences
Blood Chemical Analysis - economics
Blood Chemical Analysis - methods
Chemistry, Clinical - economics
Chemistry, Clinical - methods
Clinical laboratory techniques
Ethylenediamines - chemistry
Humans
Inflammation
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Nitrates - blood
Nitric oxide
Nitrites - blood
Oxidative stress
Reference Values
Reproducibility of Results
Serum - chemistry
Sulfanilamides - chemistry
Vasodilation
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Summary:We described an automated technique for measurement of serum nitrite/nitrate (NO x ) using the Cobas Mira clinical chemistry analyzer. NO x was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed. The automated technique for measurement of serum NO x was linear, precise, and accurate. It has a LLOQ of 2.0 μmol/L. Serum NO x measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2010.12.011