T24. The effects of low-frequency repetitive transcranial magnetic stimulation on the local field potentials of hippocampus in rats

• Published in: Clinical neurophysiology 2018-05, Vol.129, p.e10-e11
• Main Authors: Wang, Xiaoming, Pu, Tianqiang, Shu, Peng, Hou, Qianmei, Tan, Juan, Jiang, Guohui
• Format: Article
• Language: English
• ISSN: 1388-2457; 1872-8952
• DOI: 10.1016/j.clinph.2018.04.025

Repeated transcranial magnetic stimulation (rTMS), as a non-invasive brain stimulation technique, has been widely used applied in the field of neurology, psychology, rehabilitation. Previous studies have shown that low frequency rTMS have good anti-epilepsy effects, but its mechanism is not fully understood. We used the multi-channel in vivo recording technique to observe the effects of low frequency rTMS on the local field potential (LFPs) of rats hippocampal, and investigate the electrophysiological mechanism of low frequency rTMS in anti-epilepsy. Sprague-Dawley rats were randomly divided into rTMS group, control group and sham group, respectively variously treating with 500 pulses of 0.5 Hz rTMS for continuous 7 days. After last stimulation, LFPs of hippocampus were measured 20 min under anesthesia, then pilocarpine induced-epileptic seizures and recorded LFPs of hippocampus to 1 h after Status epilepticus (SE). LFPs was respectively partitioned using the common physiological frequency bands: delta (0.5–3.5 Hz), theta (4–7 Hz), gamma (30–80 Hz) and ripple (100–250 Hz), both was estimated with spectrum analysis and power spectral density. The results of spectrum analysis showed that compared with control group and sham group, low-frequency rTMS markedly increased spectrum energy and the power spectral density of delta, theta and gamma-band and significantly decreased the value of ripple oscillation (P