Designing of ω-3 PUFA enriched biocompatible nanoemulsion with sesame protein isolate as a natural surfactant: Focus on enhanced shelf-life stability and biocompatibility

[Display omitted] Natural polymeric proteins, isolated from sesame meal, had already shown promise as potent nutraceutical. This polymeric protein was employed as a natural alternative surfactant for designing a nanoemulsion based delivery system for ω-3 PUFA rich fish oil (FO) in the present study....

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Bibliographic Details
Published in:Colloids and surfaces. A, Physicochemical and engineering aspects Physicochemical and engineering aspects, 2018-02, Vol.538, p.36-44
Main Authors: Dey, Tanmoy Kumar, Banerjee, Priya, Chatterjee, Roshni, Dhar, Pubali
Format: Article
Language:English
Subjects:
Biocompatibility
High pressure homogenization
MTT assay
Nanoemulsion
Omega-3 PUFA
PBMCs
Release kinetics
Sesame protein isolate
Shelf-life stability
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Summary:[Display omitted] Natural polymeric proteins, isolated from sesame meal, had already shown promise as potent nutraceutical. This polymeric protein was employed as a natural alternative surfactant for designing a nanoemulsion based delivery system for ω-3 PUFA rich fish oil (FO) in the present study. The process parameters of the high pressure nanoemulsification were optimized with the CCD guided RSM analysis. Results with highest desirability [cumulative droplet size 125.87±2.54nm, PdI 0.199±0.01 and D90 Particle size 268.98±13.89nm] was obtained with 40 Kpsi homogenization pressure and 10 homogenization cycles and upto 1.5% (w/v) FO. The nanoemulsion formulation with SPI at 0.5% (w/v) concentration in combination with Tween 20 and Span 80 (1:1) had minimized the hydrodynamic droplet size to 89.68±2.375nm and also enhanced the shelf-life stability of the nanoformulations substantially, along an 8 week long storage period. Inclusion of SPI as surfactant also contributed to ≥90% of fatty acid release from the fish oil rich nanoemulsion droplets, at a steady gradient during 120min of simulated two-step in vitro digestion. The MTT assay had confirmed the formulation to be non-toxic towards rat PBMCs. The informations obtained in this study will facilitate the application of SPI to formulate nutraceuticals-loaded nanoemulsion delivery system with better functional benefits.
ISSN:0927-7757
1873-4359
DOI:10.1016/j.colsurfa.2017.10.066