Insight into the composition and surface corona reliant biological behaviour of quercetin engineered nanoparticles

Quercetin can be used as the reducing and stabilizing agent during the preparation of Au/Ag nanoparticles. It imparts bioactive corona with novel characteristics. [Display omitted] Physicochemical properties of nanoparticles have noteworthy influence on their interaction with biological systems. In...

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Bibliographic Details
Published in:Colloids and surfaces. A, Physicochemical and engineering aspects Physicochemical and engineering aspects, 2018-07, Vol.548, p.1-9
Main Authors: Ugru, Medha M., Sheshadri, Sanjana, Jain, Devendra, Madhyastha, Harishkumar, Madhyastha, Radha, Maruyama, Masugi, Navya, P.N., Daima, Hemant Kumar
Format: Article
Language:English
Subjects:
Alloy nanoparticles
Biological
Composition
Cytotoxicity
Quercetin
Surface corona
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Summary:Quercetin can be used as the reducing and stabilizing agent during the preparation of Au/Ag nanoparticles. It imparts bioactive corona with novel characteristics. [Display omitted] Physicochemical properties of nanoparticles have noteworthy influence on their interaction with biological systems. In the present study, we have developed gold, silver and their bimetallic alloy nanoparticles of different compositions using plant-derived aglycone flavonoid quercetin as a reducing and stabilizing agent. It is interesting that a simple biomolecule can provide a handle to control the composition in a facile manner besides suitable biomolecular corona. Moreover, the presence of quercetin corona in conjugation with specific metal composition exhibited in-vitro radical scavenging capabilities, inherent in-vitro peroxidase like behaviour and antibacterial activities in selective manner. In addition, the cytotoxic effects of these nanoparticles on skin fibroblast cells (M5S) were assessed by membrane leakage of lactate dehydrogenase (LDH), reactive oxygen species (ROS) generation and cellular morphology analysis. All the nanoparticles were found to have composition and surface corona reliant effects on M5S cells. Furthermore, quercetin capping increased cell number and induced the collagen type-1A mRNA expression, confirming collagen synthesis in cells upon treatment. This study reveals that the investigated biological actions of engineered nanoparticles are driven by metal composition of nanoparticles in addition to their surface corona; and fine-tuning of these properties can open new opportunities for a wide range of applications.
ISSN:0927-7757
1873-4359
DOI:10.1016/j.colsurfa.2018.03.055