Correlation between Cells-on-Chips materials and cell adhesion/proliferation focused on material’s surface free energy

[Display omitted] Cell surface reactions such as material surface characteristics and cell proliferation/adhesion rates are correlated and this correlation can be used as early determination criteria for shortening the development period and reducing costs of selecting materials used for bio-experim...

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Bibliographic Details
Published in:Colloids and surfaces. A, Physicochemical and engineering aspects Physicochemical and engineering aspects, 2019-03, Vol.565, p.188-194
Main Authors: Ueno, Hidetaka, Inoue, Masatoshi, Okonogi, Atsuhito, Kotera, Hidetoshi, Suzuki, Takaaki
Format: Article
Language:English
Subjects:
Adhesion rate
Biocompatibility
Cells-on-Chips
Microelectromechanical systems
Proliferation rate
Surface free energy
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Summary:[Display omitted] Cell surface reactions such as material surface characteristics and cell proliferation/adhesion rates are correlated and this correlation can be used as early determination criteria for shortening the development period and reducing costs of selecting materials used for bio-experiments, and of bio-experimental instruments. When fabricating Cells-on-Chips, a microfluidic device fabricated using MEMS technology, the influence of the scaffold material should be considered. Since a wide variety of materials are used as constituent materials of Cells-on-Chips, highly versatile early determination criteria corresponding to a specific environment and various materials are needed. In this research, we propose the early determination criteria of scaffolding material composing Cells-on-Chips by using surface free energy, which is calculated from contact angle that can be measured easily and inexpensively. Six materials were selected, regardless of inorganic/organic and photosensitive/non-photosensitive properties. When HeLa cells were cultured on each material, a significant correlation was observed between surface free energy and cell proliferation/adhesion rates. Therefore, the surface free energy of the material is appropriate as the early determination criteria for selecting the constituent material of Cells-on-Chips. Compared with the conventional criteria, the proposed criteria have high versatility regardless of the kind of material and can be used for evaluation of various materials.
ISSN:0927-7757
1873-4359
DOI:10.1016/j.colsurfa.2018.12.059