Synergistic catalysis of immobilized enzyme and peroxymonosulfate activation: CuFe2O4/kaolin-CTS-Laccase for enhanced performance of reactive blue 19 degradation

• Published in: Colloids and surfaces. A, Physicochemical and engineering aspects Physicochemical and engineering aspects, 2025-02, Vol.706, p.135784, Article 135784
• Main Authors: Jing, Lingyun, Li, Xinyong, Chen, Jixiang, Quan, Rui, Wang, Tong, Zhao, Xia, Yang, Wenhan, Liu, Shasha, Hu, Yeqiang, Sun, Zhili, Hao, Pengbo, Yang, Hui, Zheng, Yinqin, Zhu, Hao
• Format: Article
• Language: English
• Subjects: CuFe2O4/kaolin; Immobilized laccase; Peroxymonosulfate; Reactive blue 19; Synergistic catalysis
• ISSN: 0927-7757
• DOI: 10.1016/j.colsurfa.2024.135784

This work constructed an immobilized laccase - peroxymonosulfate (PMS) activation synergistic catalytic system for enhancing the efficient removal of reactive blue 19 (RB19). Superparamagnetic CuFe2O4/kaolin (CF/K) composites were prepared by one-step solvothermal and calcination methods and then modified with chitosan as immobilized carriers for laccase. The enzyme activity of CuFe2O4/kaolin-CTS-Laccase (CF/K-CTS-Lac) could reach 316.3 U/g under optimal immobilization conditions of 3 h and 2.6 mg/mL. CF/K-CTS-Lac exhibited improved pH stability and thermal stability in comparison to free laccase. The CF/K-CTS-Lac/PMS synergistic catalytic system achieved 98.4 % decolourization of RB19 within 90 min, which was 1.7 and 1.9 times higher than that of the CF/K-CTS-Lac system and CF/K-CTS/PMS system, respectively. Enhanced RB19 degradation efficiency attributed to synergistic catalysis by immobilized laccase and PMS activation. Free radical quenching experiments showed that hydroxyl radicals (•OH), sulfate radicals (SO4•-), superoxide radicals (•O2-) and singlet oxygen (1O2) were involved in the synergistic catalytic system, with •O2- and 1O2 playing a major role. Our investigations are expected to provide new insights into the construction of synergistic catalytic systems for pollutants. [Display omitted] •Immobilized laccase - PMS activation synergistic catalytic system was constructed.•Laccase and CuFe2O4 nanoparticles were uniformly and firmly dispersed on kaolin.•CuFe2O4/kaolin-CTS-Laccase demonstrates superior sustainability and catalytic activity.••OH, SO4•-, •O2- and 1O2 are involved in synergistic catalytic degradation of RB19.