Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: In situ atomic force microscopy and nanolithography study

[Display omitted] ► Nanobubbles and nanopancakes influence the protein adsorption. ► Atomic force nanolithography allows the patterning of protein layer. ► Fabrication of gaseous nanocavities of controlled dimensions. Air nanobubbles and nanopancakes were investigated in situ by both tapping mode at...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-06, Vol.94, p.213-219
Main Authors: Kolivoška, Viliam, Gál, Miroslav, Hromadová, Magdaléna, Lachmanová, Štěpánka, Tarábková, Hana, Janda, Pavel, Pospíšil, Lubomír, Turoňová, Andrea Morovská
Format: Article
Language:English
Subjects:
Air
Animals
Atomic force microscopy
Atomic force nanolithography
bovine serum albumin
Cattle
colloids
denaturation
Ethanol
Gases
graphene
Graphite - chemistry
Highly ordered pyrolytic graphite
Microscopy, Atomic Force
Nanobubbles
nanomaterials
Nanopancakes
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology
Protein adsorption
Serum Albumin, Bovine - chemistry
Surface Properties
Water
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Summary:[Display omitted] ► Nanobubbles and nanopancakes influence the protein adsorption. ► Atomic force nanolithography allows the patterning of protein layer. ► Fabrication of gaseous nanocavities of controlled dimensions. Air nanobubbles and nanopancakes were investigated in situ by both tapping mode atomic force microscopy (TM AFM) and atomic force nanolithography techniques employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange served for conservation of nanobubble and nanopancake sites appearing as imprints in BSA film left by gaseous cavities formerly present on the interface in the aqueous environment. Once the BSA film was gently removed by the nanoshaving technique applied in ethanol, a clean basal plane HOPG area with well-defined dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led to the re-formation of nanopancakes specifically at the nanoshaved area. Our approach paves the way for the study of gaseous nanostructures with defined dimensions, formed at solid–liquid interface under controlled conditions.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2012.01.028