Structural characterization and antimicrobial properties of silver nanoparticles prepared by inverse microemulsion method

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-01, Vol.101, p.243-250
• Main Authors: Wani, Irshad A., Khatoon, Sarvari, Ganguly, Aparna, Ahmed, Jahangeer, Ahmad, Tokeer
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Candida albicans - drug effects; Candida albicans - growth & development; Cetrimonium Compounds - chemistry; Emulsions; Escherichia coli - drug effects; Escherichia coli - growth & development; Microbial Sensitivity Tests; Microemulsion; Microscopy, Electron, Transmission; Nanoparticles - chemistry; Octoxynol - chemistry; Particle Size; Poloxalene - chemistry; Silver - chemistry; Silver - pharmacology; Silver nanoparticles; Spectrophotometry, Ultraviolet; Surface area and antimicrobial properties; Surface Plasmon Resonance; Surface-Active Agents - chemistry; Transmission electron microscopy; X-Ray Diffraction
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2012.07.001

Silver nanoparticles with size in the range of 40–8nm synthesized inside microemulsions formed by the cationic and nonionic surfactants. The surface area increases with decrease in the particle size. The silver nanoparticles show potent biocidal action against the test microbes. [Display omitted] ► Systematic study of the effect of the surfactants on the particle size and properties of the silver nanoparticles. ► Uniform and monodisperse silver nanoparticles (8–40nm). ► Promising optical and surface area properties. ► Excellent antimicrobial activity of silver nanoparticles against the test microbes. Silver nanoparticles have been synthesized in the inverse microemulsions formed using three different surfactants viz., cetyl-trimethyl ammonium bromide (CTAB), Tergitol and Triton X-100. We have done a systematic study of the effect of the surfactants on the particle size and properties of the silver nanoparticles. Microscopic studies show the formation of spheres, cubes and discs shaped silver nanostructures with the size in the range from 8 to 40nm. Surface plasmon resonance (SPR) peak was observed around 400nm and 500nm. In addition to SPR some extra peaks have also been observed due to the formation of silver metal clusters. The surface area increases from 3.45 to 15.06m2/g with decreasing the size of silver nanoparticles (40–8nm). To investigate the antimicrobial activity of silver nanoparticles, the nanoparticles were tested against the yeast, Candida albicans and the bacterium, E. coli. The results suggest very good antimicrobial activity of the silver nanoparticles against the test microbes. The mode of action of the antimicrobial activity was also proposed.