One pot synthesis of gold nanoparticles and application in chemotherapy of wild and resistant type visceral leishmaniasis

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-07, Vol.107, p.27-34
• Main Authors: Das, Suvadra, Roy, Partha, Mondal, Subhasish, Bera, Tanmoy, Mukherjee, Arup
• Format: Article
• Language: English
• Subjects: Animals; Antiprotozoal Agents - pharmacology; Antiprotozoal Agents - therapeutic use; colloids; crystal structure; drug resistance; Drug Resistance - drug effects; Drug Therapy; drugs; Flavonoid; Fourier transform infrared spectroscopy; gold; Gold - pharmacology; Gold - therapeutic use; Gold nanoparticle; Humans; inhibitory concentration 50; Leishmania donovani; Leishmaniasis, Visceral - drug therapy; Leishmaniasis, Visceral - parasitology; macrophages; Macrophages - drug effects; Macrophages - parasitology; Macrophages - ultrastructure; Metal Nanoparticles - therapeutic use; Metal Nanoparticles - ultrastructure; nanogold; nanoparticles; Nanotechnology - methods; parasites; Quercetin; Quercetin - pharmacology; Quercetin - therapeutic use; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared; temperature; toxicity; transmission electron microscopy; Visceral leishmaniasis; World Health Organization; X-Ray Diffraction
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2013.01.061

[Display omitted] ► One pot synthesis of gold nanoparticles using flavonoid compound quercetin. ► Functionalized gold particles were confirmed in TEM FTIR UV XRD and EDAX experiments. ► Nanoparticle efficacy was significant in wild and drug resistant leishmaniasis. ► Macrophage uptake of gold nanoparticles was confirmed in TEM. Gold nanoparticles (Aunp) through biogenetic processes have induced enormous interest for lower toxicity and precise applications. A rapid, one pot synthesis for uniformly sized gold nanoparticles was developed using polyphenolic compound quercetin. Reduction process was followed at low temperatures in a simple bath type sonicator. Nanoparticle plasmon response was recorded at 540nm and the average size in TEM was observed at 15.07nm. Detailed X-ray diffraction (XRD) observations proved fcc crystalline structure of metallic gold and the Fourier transform infrared (FTIR) analysis has confirmed nanoparticles conjugation with quercetin. Leishmaniasis, is a neglected tropical disease (NTD) classified by the World Health Organization (WHO). The leishmanial parasite multiply in host macrophages and most strains have developed drug resistance to available chemotherapeutics. Drug delivery is therefore a major problem in macrophage specific leishmanial parasite infections. New quercetin conjugated gold nanoparticles (QAunp) were successfully evaluated for the first time against leishmanial macrophage infections. Antileishmanial efficiency of QAunp was established against wild type (IC50 15±3), sodium stibogluconate resistant strain (IC50 40±8) and the paramomycin resistant (IC50 30±6) strains. Macrophage uptake of QAunp was complete within an hour as observed in TEM experiments.