Biomimetic nanomaterials: Development of protein coated nanoceria as a potential antioxidative nano-agent for the effective scavenging of reactive oxygen species in vitro and in zebrafish model

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2016-10, Vol.146, p.375-386
• Main Authors: Bhushan, Bharat, Nandhagopal, Soundharapandiyan, Rajesh Kannan, Rajaretinam, Gopinath, P.
• Format: Article
• Language: English
• Subjects: Albumin; Albumins - chemistry; Animals; Antioxidant; Antioxidants - chemistry; Antioxidants - pharmacology; Biomimetic nanomaterials; Biomimetics; Cell Survival - drug effects; Cells, Cultured; Cerium - chemistry; Cerium - pharmacology; Embryo, Nonmammalian - cytology; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - metabolism; Humans; In Vitro Techniques; Lung - cytology; Lung - drug effects; Lung - metabolism; Models, Animal; Nanoceria; Nanostructures - administration & dosage; Nanostructures - chemistry; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism; Zebrafish
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2016.06.035

Antioxidant potentials of protein coated nanoceria against H2O2 induced oxidative stress have been examined both in human lung epithelial cells and in zebrafish. [Display omitted] ⿢Synthesized albumin coated nanoceria (ANC) particles with antioxidant activity.⿢ANC was characterised by various physico-chemical methods.⿢Enzyme mimetic activity of ANC was confirmed by antioxidant enzyme assays.⿢Biocompatibility and antioxidant potential of ANC was assessed in vitro.⿢Antioxidant potential of ANC has been realized for the first time against zebrafish. Reactive oxygen species (ROS) induced oxidative stress is one of the major factors responsible for initiation of several intracellular toxic events that leads to cell death. Antioxidant enzymes defence system of the body is responsible for maintaining the oxidative balance and cellular homeostasis. Several diseases are promoted by the excessive oxidative stress caused by the impaired antioxidant defence system that leads to oxidant/antioxidant imbalance in the body. In order to restore or precise the aberrant antioxidant system, a large number of catalytic nanoparticles has been screened so far. Exceptional antioxidative activity of nanoceria made it as a potential antioxidative nano-agent for the effective scavenging of toxic ROS. In this work albumin coated nanoceria (ANC) was synthesized and further characterised by various physicochemical techniques. The antioxidant and superoxide dismutase (SOD) assay confirm that the albumin coating do not alter the antioxidant potential of ANC. The biocompatibility and protective efficacy of ANC against oxidative stress was investigated both in vitro and in vivo in human lung epithelial (L-132) cells and zebrafish embryos, respectively. The inductively coupled plasma mass spectrometry (ICP-MS), transmission electron microscopy (TEM) and field emission scanning electron microscope (FE-SEM) analysis corroborates the uptake of ANC by the cells. Furthermore, the semi-quantitative gene expression studies confirmed that the ANC successfully defend the cells against oxidative stress by preserving the antioxidant system of the cells. Thus, the current work open up a new avenue for the development of improved antioxidant nano-drug therapies.