EGCG modified small intestine submucosa promotes wound healing through immunomodulation

• Published in: Composites. Part B, Engineering Engineering, 2023-12, Vol.267, p.111005, Article 111005
• Main Authors: Nie, Rong, Zhang, Qing-Yi, Tan, Jie, Feng, Zi-Yuan, Huang, Kai, Sheng, Ning, Jiang, Yan-Lin, Song, Yu-Ting, Zou, Chen-Yu, Zhao, Long-Mei, Li, He-Xi, Wang, Rui, Zhou, Xing-Li, Hu, Juan-Juan, Wu, Chen-Yu, Li-Ling, Jesse, Xie, Hui-Qi
• Format: Article
• Language: English
• Subjects: Epigallocatechin gallate; Immune microenvironment; Small intestinal submucosa; Wound healing
• ISSN: 1359-8368; 1879-1069
• DOI: 10.1016/j.compositesb.2023.111005

Decellularized porcine small intestinal submucosa (SIS) has shown promising therapeutic efficacy as a functional wound dressing. Nevertheless, its limited anti-oxidative and immunomodulatory capacities have restricted its application for the treatment of complex skin wounds. Herein, epigallocatechin gallate (EGCG), a polyphenolic compound, was employed for the modification of the SIS to overcome such shortcomings. The EGCG-modified SIS (E-SIS) has shown excellent biocompatibility and improved hydrophilicity for cell adhesion. Notably, in vitro studies showed that the E-SIS could effectively alleviate oxidative stress and facilitate the M1-to-M2 phenotype transition of macrophages, thereby creating a favorable immune microenvironment for cell proliferation, migration, collagen synthesis as well as angiogenesis. A full-thickness skin defect model, combined with macrophage depletion, has further confirmed that the E-SIS could accelerate skin wound repair through immunomodulation in vivo. This suggested that the EGCG modification could provide a facile yet effective method to broaden the applications of the SIS for skin wound management.