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Study on improving the consolidation properties of microbial cementitious material by promoting spore germination ratio

•Evaluation of spore germination was investigated by the absorbance difference.•The effect of heat shock, germination agent and nutriment on spore germination was optimized.•Effect of spore dosage on microstructure and mechanical properties was determined. Microbial cementitious material, as a new t...

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Bibliographic Details
Published in:Construction & building materials 2020-08, Vol.252, p.119036, Article 119036
Main Authors: Zhan, Qiwei, Yu, Xiaoniu, Zhang, Shengju, Xu, Ying, Pan, Zhihong, Qian, Chunxiang
Format: Article
Language:English
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Summary:•Evaluation of spore germination was investigated by the absorbance difference.•The effect of heat shock, germination agent and nutriment on spore germination was optimized.•Effect of spore dosage on microstructure and mechanical properties was determined. Microbial cementitious material, as a new type of green environmental protection cementitious material, was getting more and more attention. In this paper, improving the consolidation properties of microbial cementitious material by promoting spore germination ratio was studied systematically. Evaluation of spore germination was investigated by the absorbance difference between spores and vegetative bodies, and the relationship between absorbance of microbial solution and spore germination ratio was determined. The effect of heat shock, germination agent and nutriment on spore germination was optimized. The results displayed that the suitable heat shock temperature was 70 °C for 10 min, and the optimum germination agent was alanine with 6 mmol/l of concentration, while nutrient could not effectively improve the spore germination ratio. Effect of spore dosage on microstructure and mechanical properties of consolidation sand were also determined. Compared with the control group, consolidation properties of microbial cementitious material with 2.5 g of spores was better, and that with 2.0 g of spores was basically consistent. The results demonstrated that the spore dosage could be reduced after improving the spore germination, and the cost of microbial cementitious material was greatly declined.
ISSN:0950-0618
1879-0526
DOI:10.1016/j.conbuildmat.2020.119036