Fluorescence resonance energy transfer induced by conjugation of metalloproteins to nanoparticles

We show the possibility of realizing a hybrid system composed of a semiconductor nanoparticle (NP) and a metalloprotein, in which the photophysical properties of the two species can be exploited to elicit fluorescence resonance energy transfer mechanisms from the biomolecule to the NP. A specific co...

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Bibliographic Details
Published in:Chemical physics letters 2006-01, Vol.417 (4), p.351-357
Main Authors: Pompa, P.P., Chiuri, R., Manna, L., Pellegrino, T., del Mercato, L.L., Parak, W.J., Calabi, F., Cingolani, R., Rinaldi, R.
Format: Article
Language:English
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Summary:We show the possibility of realizing a hybrid system composed of a semiconductor nanoparticle (NP) and a metalloprotein, in which the photophysical properties of the two species can be exploited to elicit fluorescence resonance energy transfer mechanisms from the biomolecule to the NP. A specific conjugation process between CdSe/ZnS core/shell water soluble NPs, functionalized with surface exposed thiol groups (–SH), and the apo form of the metalloprotein azurin (Az) has been achieved, resulting in a fixed distance in the donor-acceptor pairs. The increase in the NP fluorescence intensity was found to be dependent on the Az to NP molar ratio.
ISSN:0009-2614
1873-4448
DOI:10.1016/j.cplett.2005.09.133