NMR investigation of the effect of caffeine on the hetero-association of an anticancer drug with a vitamin

• Published in: Chemical physics letters 2006-12, Vol.432 (1), p.248-251
• Main Authors: Evstigneev, M.P., Evstigneev, V.P., Davies, D.B.
• Format: Article
• Language: English
• ISSN: 0009-2614; 1873-4448
• DOI: 10.1016/j.cplett.2006.10.008

The complexation of anthracycline antibiotic Daunomycin and vitamin B2 derivative has been investigated by NMR in the presence of a third aromatic molecule, Caffeine. Quantitative analysis of the ternary mixture suggests that the in vivo efficacy of Drug–Vitamin interaction may be modified by the presence of caffeine. The complexation between an anti-cancer drug Daunomycin (DAU) and a Vitamin B 2 derivative, flavin-mononucleotide (FMN), in the presence of a third type of aromatic molecule, caffeine (CAF), in aqueous solution has been studied by NMR spectroscopy. Ternary mixtures of the drug, vitamin and caffeine have been analysed quantitatively taking into account all possible complexation reactions between the aromatic molecules in solution. The results show that complexation between DAU and FMN decreases on addition of CAF which suggests that caffeine at physiological concentrations in vivo may affect the biological synergism of drug and vitamin.