A real-time PCR assay for the detection of azoxystrobin-resistant Alternaria populations from pistachio orchards in California

Azoxystrobin-resistant populations of Alternaria spp. in the alternata, tenuissima, and arborescens species–groups, the causal agents of Alternaria late blight of pistachio, have been selected in pistachio orchards in California. The azoxystrobin resistance in Alternaria spp. was found to be correla...

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Bibliographic Details
Published in:Crop protection 2004-12, Vol.23 (12), p.1259-1263
Main Authors: Ma, Zhonghua, Michailides, T.J.
Format: Article
Language:English
Subjects:
Alternaria
Alternaria alternata
Alternaria arborescens
Alternaria late blight of pistachio
Alternaria tenuissima
bioassays
blight
disease detection
DNA primers
fungal diseases of plants
fungicide resistance
fungicides
gene amplification
orchards
pistachios
Pistacia vera
plant pathogenic fungi
polymerase chain reaction
QoI fungicides
Real-time PCR
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Summary:Azoxystrobin-resistant populations of Alternaria spp. in the alternata, tenuissima, and arborescens species–groups, the causal agents of Alternaria late blight of pistachio, have been selected in pistachio orchards in California. The azoxystrobin resistance in Alternaria spp. was found to be correlative to a single point mutation resulting in the replacement of a glycine by an alanine at codon 143 (G143A) in the mitochondrial cytochrome b ( cyt b) gene. Based on this point mutation, a real-time PCR method was developed to rapidly detect azoxystrobin-resistant Alternaria populations in field leaf samples collected from pistachio orchards in California.
ISSN:0261-2194
1873-6904
DOI:10.1016/j.cropro.2004.05.012