The target-site based resistance mechanism of Alopecurus myosuroides Huds. To pyroxsulam

Alopecurus myosuroides Huds. is a troublesome weed in wheat (Triticum aestivum L.) fields. In 2017, two A. myosuroides populations that survived pyroxsulam treatment at the recommended field rate were collected from winter wheat fields in China. This study was conducted to determine the resistance l...

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Bibliographic Details
Published in:Crop protection 2021-09, Vol.147, p.105707, Article 105707
Main Authors: Huang, Zhaofeng, Lu, Xingtao, Wei, Shouhui, Zhang, Chaoxian, Jiang, Cuilan, Li, Wenyu, Saeed, Muhammad, Huang, Hongjuan
Format: Article
Language:English
Subjects:
ALS enzyme
Herbicide
Herbicide resistance
Target site mutation
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Summary:Alopecurus myosuroides Huds. is a troublesome weed in wheat (Triticum aestivum L.) fields. In 2017, two A. myosuroides populations that survived pyroxsulam treatment at the recommended field rate were collected from winter wheat fields in China. This study was conducted to determine the resistance level and the basis of resistance to pyroxsulam in A. myosuroides. Sequencing of acetolactate synthase (ALS) revealed a Pro-197-Thr mutation in the HB1 population and two mutations (Pro-197-Thr or Trp-574-Leu) in the SD2 population. Purified subpopulations for the Pro-197-Thr and Trp-574-Leu mutations were produced. Whole-plant dose-response tests and in vitro ALS activity assays showed that the purified sHB1 and sSD2 populations displayed moderate resistance (
ISSN:0261-2194
1873-6904
DOI:10.1016/j.cropro.2021.105707