Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

• Published in: Cryobiology 2015-08, Vol.71 (1), p.18-23
• Main Authors: Naseer, Zahid, Ahmad, Ejaz, Aksoy, Melih, Küçük, Niyazi, Serin, İlker, Ceylan, Ahmet, Boyacıoğlu, Murat, Kum, Cavit
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Cell Membrane - drug effects; Cell Survival - drug effects; Cholesterol - metabolism; Cholesterol - pharmacology; CLC; Cryopreservation; Cryopreservation - methods; Cyclodextrins - metabolism; Cyclodextrins - pharmacology; H2O2; Humans; Hydrogen Peroxide - toxicity; Lipid Peroxidation; Male; Malondialdehyde - analysis; Oxidation-Reduction; Oxidative stress; Oxidative Stress - drug effects; Ram sperm; Semen - metabolism; Semen Preservation - methods; Sheep; Sperm Motility - drug effects; Spermatozoa - drug effects
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2015.06.007

Three experiments were conducted to determine the protective effect of cholesterol-loaded cyclodextrin (CLC) against hydrogen peroxide (H2O2) or cryo-induced damage in ram sperm. In Experiment 1, the fresh ejaculates were either treated with CLC or remained untreated. Both CLC treated and untreated samples were then incubated with 0, 250 or 500μM H2O2 at 35°C for 12h. After incubation period of 12h, the motility, viability and membrane integrity remained higher in CLC treated sperm even in the presence of 250 or 500μM H2O2. The H2O2 treatment affected all the sperm parameters adversely (P