P105 Effects of Azardirchata indica and Acatia nilotica extracts on splenocytes proliferation and IFN-Γ cytokine induction in wistar albino rats

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2012-09, Vol.59 (3), p.553-553
• Main Authors: Singh, B., Yadav, S.K., Kamal, Pradeep K., Singh, Rashmi, Gupta, Jayati, Bashir, Shahnaz, Srivastav, Abhishek
• Format: Article
• Language: English
• Subjects: Ayurvedic medicine; B-lymphocytes; cell differentiation; cell proliferation; cell suspension culture; cytokines; disease resistance; epidemiology; herbal medicines; immunomodulators; mice; neutralization; pathogens; rats; secretion; spleen; splenocytes; t-test
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2012.06.196

One of the therapeutic strategies in Ayurvedic medicine is to increase body’s natural resistance to the disease causing agent rather than directly neutralizing the agent it self. This concept in modern scientific understanding would mean enhancement of immune responsiveness of an animal against pathogens by nonspecifically activating the immune system using immunomodulatory agents of plant origin. Use of herbal medicines, as immunomodulators, which alter the activity of immune function through the dynamic regulation of informational molecules such as cytokines. Modulation of cytokines’ secretions may offer novel approaches in the treatment of variety of diseases.This study was designed to investigate the effect of A.indica and A.nilotica on cytokine induction and splenocyte proliferation to establish the concept. MDBK Cell line was already maintained in the Animal Cell Culture facility under Niche Area of Excellence Project, Department of Epidemiology, DUVASU, Mathura. The cell lines were grown in RPMI 1640 and incubated at 37°C in 5% CO2.The animals were obtained from Animal House facility at DUVASU, Marthura. The spleens were aseptically removed, and placed in RPMI 1640. Single cell suspensions were prepared and splenocytes were cultured with sample and mitogen or without mitogen for specific cell differentiation to T and B lymphocytes (Asia et al.). The cell proliferation was measured by MTT assay.Cell proliferation was expressed as percentage of viable cells of treated samples to control samples. Concentration of IFN-Gamma was measured using the commercially available ELISA kits according to the protocol provided by the manufacturer. Data processsed for statistical analysis using student t-test. In vitro Effect of aq. Extract of A.indica and A.niloticaleaves on splenocyte proliferation: The mean value for splenocyte proliferation using four different concentration (62.5, 125, 250 & 500μg/well) of A. indica were 0.120±0.001, 0.115±0.004, 0.089±0.005 & 0.073 respectively The mean value of control was 0.112±0.002. The result showed the maxium inhibition in splenocyte proliferation in spleen cells harvested from normal mice treated with 250μg/well and 500μg/well A. indica extract which were 20.53% and 34.82% respectively. While as The mean value for splenocyte proliferation using four different concentration (62.5, 125, 250 & 500μg/well) of A. nilotica were 0.085±0.002, 0.054±0.001, 0.015±0.001 and 0.013±0.00 respectively. The mean value of control was 0.1