Identification of Streptococcus pseudopneumoniae and other mitis group streptococci using matrix assisted laser desorption/ionization - time of flight mass spectrometry

This study evaluated the ability of the MALDI-ToF MS from Bruker Daltonics to identify clinical Mitis-Group-Streptococcus isolates with a focus on Streptococcus pseudopneumoniae. The results were analyzed using the standard log(score) and the previously published list(score). Importantly, using the...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2021-11, Vol.101 (3), p.115487, Article 115487
Main Authors: Jensen, Christian Salgård, Dargis, Rimtas, Shewmaker, Patricia, Nielsen, Xiaohui Chen, Christensen, Jens Jørgen
Format: Article
Language:English
Subjects:
Genome, Bacterial
Humans
MALDI-ToF MS
Mitis Group
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Streptococcus
Streptococcus - chemistry
Streptococcus - classification
Streptococcus - genetics
Streptococcus - isolation & purification
Streptococcus pneumoniae
Streptococcus pseudopneumoniae
Viridans Streptococci - chemistry
Viridans Streptococci - classification
Viridans Streptococci - genetics
Viridans Streptococci - isolation & purification
Whole Genome Sequencing
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Summary:This study evaluated the ability of the MALDI-ToF MS from Bruker Daltonics to identify clinical Mitis-Group-Streptococcus isolates with a focus on Streptococcus pseudopneumoniae. The results were analyzed using the standard log(score) and the previously published list(score). Importantly, using the log(score) no misidentifications occurred and 27 of 29 (93%) S. pneumoniae and 27 of 30 (90%) S. oralis strains were identified, but only 1 of 31 (3%) S. pseudopneumoniae and 1 of 13 (8%) S. mitis strains were identified. However, our results show that 30 of 31 S. pseudopneumoniae strains had a S. pseudopneumoniae Main Spectral Profiles within the 3 best matches. Using the list(score) all S. oralis and S. pneumoniae strains were identified correctly, but list(score) misidentified 10 S. pseudopneumoniae and 5 S. mitis. We propose to use the log(score) for identification of S. pneumoniae, S. pseudopneumoniae, S. mitis and S. oralis, but for some strains additional testing may be needed.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2021.115487