Invitro evaluation of piperine enclosed erythrocyte carriers

• Published in: Drug invention today 2013-09, Vol.5 (3), p.169-174
• Main Authors: Madhavi, Boddupalli Bindu, Bhavana, Madupoju, Nath, Anisetti Ravinder, Prasad, Masana, Siri Vennela, Kallem
• Format: Article
• Language: English
• Subjects: Erythrocyte carriers; Hepato toxicity; Hypotonic dilution method; Piperine
• ISSN: 0975-7619; 0975-7619
• DOI: 10.1016/j.dit.2013.04.006

Resealed erythrocytes are the promising drug delivery systems in targeting drug delivery system. In our present work, hepato protective agent piperine was encapsulated into erythrocyte carriers in order to target it to liver. Resealed erythrocytes were prepared by following hypotonic dilution technique. The effects of NaCl in hypotonic and hypertonic solutions, drug concentration, freezing and solvent were studied on encapsulation and hemoglobin release. After optimizing, the erythrocytes were evaluated for hematological parameters, osmotic shock, turbulence shock, size and invitro release studies. From the results it was found that, the prepared erythrocytes were with ≈200 μm size. After optimization of the various parameters the carrier erythrocytes were found to have higher drug content and hemoglobin release at 0.25% NaCl of hypotonic, 2% NaCl of hypertonic, 1.5% initial drug concentration and after freezing for a day. The optimized erythrocyte carriers were found to be stable for external shocks with no significant change in haemotological parameters and with first order release of piperine. The piperine enclosed carrier erythrocytes are going to be the promising option for treating hepatic toxicity. [Display omitted]