A ratiometric fluorescent probe based on phenothiazine and HBI motif for rapid detection of HClO and its applications

• Published in: Dyes and pigments 2023-01, Vol.209, p.110930, Article 110930
• Main Authors: Deng, Xuemiao, Yu, Xin, Wei, Fang, Zheng, Chunhong, Wang, Renjie, Pu, Shouzhi
• Format: Article
• Language: English
• Subjects: HBI; HClO; Paper-based sensor; Phenothiazine; Ratiometric fluorescence probe
• ISSN: 0143-7208; 1873-3743
• DOI: 10.1016/j.dyepig.2022.110930

A deep-red emissive fluorescence probe PT-HBI with phenothiazine and 4-(4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazole-5(4H)-one (HBI) motif was designed and synthesized for rapid detection of HClO in a fine ratiometric manner. PT-HBI exhibited remarkable ratiometric fluorescence response to HClO within 50 s, achieving a large Stokes shift (120 nm), high sensitivity (LOD = 0.31 μM) and excellent selectivity. Among the tested analytes, only addition HClO to PT-HBI enables result from a significant fluorescence change from red emission (λmax = 660 nm) to yellow emission (λmax = 540 nm) with the ratio of F540/F660 displaying a 175-fold enhancement. In particular, PT-HBI was capable of monitoring exogenous HClO in Hela cells by ratiometric fluorescence imaging. Moreover, the probe PT-HBI has provided an extensive application for detection of HClO in real water samples and on paper-based sensor to detect HClO in different concentration. •Probe PT-HBI displayed a large emission shift (120 nm) and a 175-fold enhancement in the ratio of F540/F660 for the detection of HClO.•Probe PT-HBI exhibited rapid response time (