Kinetic evaluation on cell growth and biosynthesis of polyhydroxybutyrate (PHB) by Bacillus safensis EBT1 from sugarcane bagasse

• Published in: Engineering in Agriculture, Environment and Food Environment and Food, 2018, Vol.11(3), pp.145-152
• Main Authors: Sakthiselvan, Punniavan, Madhumathi, Ramasamy
• Format: Article
• Language: English
• Subjects: Bacillus safensis EBT1; Leudeking-Piret; Logistic; Nanoparticles; Polyhydroxybutyrate (PHB)
• ISSN: 1881-8366; 1881-8366
• DOI: 10.1016/j.eaef.2018.03.003

Polyhydroxybutyrate (PHB), a biodegradable polymer (an intracellular product) was produced using Bacillus safensis EBT1. A kinetic model that describes microbial growth, biopolymer production and substrate consumption was used to predict the performance of batch fermentation of Bacillus safensis EBT1. The experimental data were fitted with modified logistic equation. The specific growth rate (μmax) and half saturation constant (Ks) values for the models such as Monod, Contois and Herbert were found to be 0.16, 0.15, 0.13 h−1 and 79.51, 68.19, 43.39 g/L respectively. Leudeking-Piret kinetics indicated that PHB formation in this study as growth associated. The recovered polymer was characterized using Transmission Electron Microscopy, Differential Scanning Calorimetry, Thermogravimetry analysis and Nuclear Magnetic Resonance. The PHB produced was used to synthesize nanoparticles using modified nano-precipitation method. •PHB production was carried out in batch fermentation by Bacillus safensis EBT1.•Sequence alignments provide a powerful way to compare novel sequences with previously characterized genes.•Evaluation of growth kinetics for PHB production by various models.•PHB production kinetics were performed by Leudeking-Piret Model.•PHB nanoparticles were synthesized using modified method called nano-precipitation.