Assessment of domestic landfill leachate toxicity to the Asian clam Corbicula fluminea via biomarkers

• Published in: Ecotoxicology and environmental safety 2014-05, Vol.103, p.17-23
• Main Authors: Oliveira, Luciana F, Silva, Sandra M C P, Martinez, Claudia B R
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarkers - analysis; Biotransformation; Bivalve; Corbicula - drug effects; Cytochrome P-450 CYP1A1 - metabolism; Ecotoxicology, biological effects of pollution; Electric Conductivity; Fresh water environment; Fundamental and applied biological sciences. Psychology; Gills - drug effects; Glutathione Transferase - metabolism; Hydrogen-Ion Concentration; Lipid peroxidation; Lipid Peroxidation - drug effects; Metallothionein; Metallothionein - analysis; MXR mechanism; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - chemistry; Water Pollutants, Chemical - toxicity
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2014.01.034

In order to evaluate the effects of domestic landfill leachate to bivalves Corbicula fluminea, clams were exposed to different leachate concentrations (v/v): 2, 3, 6 and 10 percent, corresponding to dilutions observed along a stream that receives this effluent, or only to clean water for comparisons. After 5 and 15 days of exposure the activity of the biotransformation enzymes 7-ethoxyresorufin-O-deethylase (EROD) and glutathione S-transferase (GST), the multixenobiotic resistance mechanism (MXR) and lipid peroxidation (LPO) in gills and digestive gland and metallothionein (MT) content in gills were evaluated. Differences in biomarkers responses were observed between gills and digestive gland, except for MXR that decreased in both tissues of clams exposed to 6 percent for 5 days. EROD activity in gills was reduced in all leachate concentrations after 5 days and only in 2 percent after 15 days exposure, while an EROD increase was observed in digestive gland after 15 days exposure to 6 percent. GST activity increased only in the gills of clams exposed to 10 percent for 5 days. LPO varied between tissues and different conditions. A significant increase in LPO was observed in the gills, after 5 days exposure to 2 and 6 percent, and in digestive gland after 5 and 15 days exposure to 2 and 3 percent. MT content in the gills increased after 15 days exposure to 2 percent. In conclusion, different leachate concentrations tested here caused biochemical changes in C. fluminea, but due to the observed variability in biomarkers responses among leachate concentrations, it was difficult to determine patterns or thresholds concentrations. •Biomarkers were measured in C. fluminea to study the toxicity of a landfill leachate.•Landfill leachate caused impairment in gills’ biotransformation process.•Lowest leachate concentration (2 percent v/v) leads to an increase in LPO and MT content.•Just in higher concentrations the activities of biotransformation enzymes increased.