HSP70 expression in Biomphalaria glabrata snails exposed to cadmium

In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96h to CdCl2 at concentrations ranging from 0.09 to 0.7mgL−1 (LC50/96h=0.34 (0.30–0.37). Time and concentration-dependent inc...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety 2017-06, Vol.140, p.18-23
Main Authors: da Silva Cantinha, Rebeca, Borrely, Sueli Ivone, Oguiura, Nancy, de Bragança Pereira, Carlos Alberto, Rigolon, Marcela M., Nakano, Eliana
Format: Article
Language:English
Subjects:
Acquired resistance
Animals
Bioindicator
Biomarker
Biomphalaria - drug effects
Biomphalaria - metabolism
Cadmium - metabolism
Cadmium - toxicity
Dose-Response Relationship, Drug
Drug Tolerance
Ecotoxicology
Environmental Monitoring - methods
Fresh Water - chemistry
Heat-Shock Response
Hot Temperature
HSP70 Heat-Shock Proteins - biosynthesis
HSP70 Heat-Shock Proteins - metabolism
Mollusk
Oxidative Stress - drug effects
Protein induction
Reproduction - drug effects
Water Pollutants, Chemical - metabolism
Water Pollutants, Chemical - toxicity
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Summary:In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96h to CdCl2 at concentrations ranging from 0.09 to 0.7mgL−1 (LC50/96h=0.34 (0.30–0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies. [Display omitted] •Time and concentration-dependent induction of HSP70 was described.•Time course of HSP70 expression was evaluated in B. glabrata.•Cross-resistance was evidenced by the protective effect of cadmium against a lethal heat shock.•This is the first data on the induction of HSP70 in Biomphalaria genus.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2017.02.026