Bioremediation of chlorpyrifos contaminated soil by two phase bioslurry reactor: Processes evaluation and optimization by Taguchi's design of experimental (DOE) methodology

• Published in: Ecotoxicology and environmental safety 2018-04, Vol.150, p.305-311
• Main Authors: Pant, Apourv, Rai, J.P.N.
• Format: Article
• Language: English
• Subjects: Biodegradation; Biodegradation, Environmental; Bioreactor; Bioreactors; Chlorpyrifos; Chlorpyrifos - analysis; Chlorpyrifos - metabolism; Design of experiments (DOE); Models, Theoretical; Orthogonal array (OA); Oxygen; Research Design; Soil - chemistry; Soil Pollutants - analysis; Soil Pollutants - metabolism; Temperature; Water
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2017.12.052

Two phase bioreactor was constructed, designed and developed to evaluate the chlorpyrifos remediation. Six biotic and abiotic factors (substrate-loading rate, slurry phase pH, slurry phase dissolved oxygen (DO), soil water ratio, temperature and soil micro flora load) were evaluated by design of experimental (DOE) methodology employing Taguchi's orthogonal array (OA). The selected six factors were considered at two levels L-8 array (2^7, 15 experiments) in the experimental design. The optimum operating conditions obtained from the methodology showed enhanced chlorpyrifos degradation from 283.86µg/g to 955.364µg/g by overall 70.34% of enhancement. In the present study, with the help of few well defined experimental parameters a mathematical model was constructed to understand the complex bioremediation process and optimize the approximate parameters upto great accuracy. •Optimization of processes parameters by Taguchi’s OA.•Design of two phase bioslurry reactor for chlorpyrifos degradation.•Comparison between generalized and optimized conditions for biodegradation.•Impact of individual, coupled and pooled factors in biodegradation.