A non-toxic microbial surfactant from Marinobacter hydrocarbonoclasticus SdK644 for crude oil solubilization enhancement

This study aims to investigate the ability of a biosurfactant produced by Marinobacter hydrocarbonoclasticus strain SdK644 isolated from hydrocarbon contaminated sediment to enhance the solubilization rate of crude oil contaminated seawater. Phylogenetic analysis shows that strain SdK644 was very cl...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety 2018-06, Vol.154, p.100-107
Main Authors: Zenati, Billal, Chebbi, Alif, Badis, Abdelmalek, Eddouaouda, Kamel, Boutoumi, Hocine, El Hattab, Mohamed, Hentati, Dorra, Chelbi, Manel, Sayadi, Sami, Chamkha, Mohamed, Franzetti, Andrea
Format: Article
Language:English
Subjects:
Artemia bioassay
Biosurfactant
Crude oil solubilization
Marinobacter hydrocarbonoclasticus
Waste frying oil
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Summary:This study aims to investigate the ability of a biosurfactant produced by Marinobacter hydrocarbonoclasticus strain SdK644 isolated from hydrocarbon contaminated sediment to enhance the solubilization rate of crude oil contaminated seawater. Phylogenetic analysis shows that strain SdK644 was very closely related to M. hydrocarbonoclasticus with 16S rRNA gene sequence similarity of 97.44%. Using waste frying oil as inducer carbon source, the producing biosurfactant by strain SdK644 was applied to improve crude oil solubilization in seawater. The preliminary characterization of the produced biosurfactant by FT-IR analysis indicates its possible classification in a glycolipids group. Results from crude oil solubilization assay showed that SdK644 strain biosurfactant was 2-fold greater than Tween 80 surfactant in crude oil solubilization and 12-fold higher than seawater control, as shown by GC-MS analysis of aliphatic compounds. Furthermore, this bioactive compound was shown to be nontoxic against Artemia larvae in short-term acute toxicity bioassay. Generally, the results showed the possible use of M. hydrocarbonoclasticus strain SdK644 biosurfactant in bioremediation processes of the marine environments. [Display omitted] •Isolation of Marinobacter hydrocarbonoclasticus SdK644 strain from Bou-Ismail Bay, Algeria.•Efficacy of biosurfactant produced by SdK644 strain to enhance crude oil solubilization in seawater.•Nontoxic character of the produced biosurfactant against Artemia animals.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2018.02.032