Natural ecotype of Arabidopsis thaliana (L.) Heynh (Chernobyl-07) respond to cadmium stress more intensively than the sensitive ecotypes Oasis and Columbia

• Published in: Ecotoxicology and environmental safety 2019-05, Vol.173, p.86-95
• Main Authors: Klimenko, Olena, Pernis, Miroslav, Danchenko, Maksym, Skultéty, Ludovít, Klubicová, Katarína, Shevchenko, Galina
• Format: Article
• Language: English
• Subjects: Annexin; Ionizing radiation; Photosynthesis; Phytochelatins; Seedling; Tolerant genotype
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2019.02.012

Large areas polluted with toxic heavy metals or radionuclides were formed as a side product of rapid industrial development of human society. Plants, due to their sessile nature, should adapt to these challenging genotoxic environmental conditions and develop resistance. Herein, we evaluated the response of three natural ecotypes of Arabidopsis thaliana (L.) Heynh (Oasis, Columbia-0, and Chernobyl-07) to cadmium, using discovery gel-based proteomics. These accessions are differing by level of tolerance to heavy metal probably achieved by various exposure to chronic ionizing radiation. Based on the pairwise comparison (control versus cadmium-treated) we recognized 5.8–13.4% of identified proteins as significantly altered at the presence of cadmium. Although the majority of photosynthesis-related proteins were found to be less abundant in all ecotypes it was noted that in contrast to the sensitive variants (Col and Oas), the tolerant Che accession may activate the mechanism preserving photosynthesis and energy production. Also, proteins modulating energy budget through alternative route and mediating higher resistance to heavy metals were upregulated in this ecotype. Although we suggest that regulation of enzymes acting in peptide and protein synthesis, protection of the plants against various abiotic stresses, or those neutralizing the effects of reactive oxygen species are rather associated with general response to cadmium, they were found to be altered more intensively in the Che accession. Thus, the identified affected proteins may represent good candidate molecules for molecular breeding to improve tolerance of crops to heavy metal stress. [Display omitted] •Response of three natural ecotypes of Arabidopsis thaliana (L.) to cadmium was evaluated.•Cadmium stress inhibits photosynthesis.•Energy production and ascorbate-glutathione cycle were primarily altered in Che accession.•Cadmium affects nitrogen and sulfur-containing aminoacid metabolic pathways.