Mechanistic elucidation of germination potential and growth of wheat inoculated with exopolysaccharide and ACC- deaminase producing Bacillus strains under induced salinity stress

• Published in: Ecotoxicology and environmental safety 2019-11, Vol.183, p.109466, Article 109466
• Main Authors: Amna, Ud Din, Bashir, Sarfraz, Sidra, Xia, Ye, Kamran, Muhammad Aqeel, Javed, Muhammad Tariq, Sultan, Tariq, Hussain Munis, Muhammad Farooq, Chaudhary, Hassan Javed
• Format: Article
• Language: English
• Subjects: ACC-Deaminase; Bacillus; Exopolysaccharide; Germination; Plant growth promoting rhizobacteria; Proline; Salt stress
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2019.109466

The potential of plant growth regulating microorganisms present in the soil can be explored towards the purpose of identifying salt tolerant strategies and crop cultivars. Current study was designed to elucidate the capabilities of salt stress tolerant plant growth promoting rhizobacteria (PGPR) Bacillus siamensis (PM13), Bacillus sp. (PM15) and Bacillus methylotrophicus (PM19) in undermining the effects of salt stress on wheat seedling. Strains were characterized for their IAA (81–113 μM/ml), ACC-deaminase (0.68–0.95 μM/mg protein/h) and exopolysaccharide (EPS) (0.62–0.97 mg/ml) producing activity both under normal and NaCl stressed conditions. Effects of bacterial inoculation on germination and seedling growth of wheat variety Pakistan-13 was observed under induced salinity stress levels (0, 4, 8, 16 dS/m). All the morpho-physiological characteristics of wheat seedlings were affected drastically by the NaCl stress and the growth parameters expressed a negative relationship with increased NaCl levels. PGPR application had a very positive influence on germination rate of wheat seedlings, root and shoot length, photosynthetic pigments etc. Elongated roots and enhanced vegetative shoot growth as well as seedling's fresh and dry weights were highest in plants treated with B. methylotrophicus PM19. Sequestration of Na+ ion by EPS production and degradation of exuded ACC into a-ketobutyrate and ammonia by ACCD bacteria efficiently reduced the impact of salinity stress on wheat growth. Current findings suggested that the used PGPR strains are potential candidates for improving crop growth in salt stressed agricultural systems. However further research validation would be necessary before large scale/field application. •Bacillus strains showed plant growth promoting traits.•Survival for various range of salinity stress.•All bacillus strains show persistent IAA, ACC deaminase and EPS production.•PGP strains enhance seedling growth, germination percentage and chlorophyll content even under high level of salinity stress.•Resistance to antibiotics revealed its potential to survive and compete native microflora in soil.