Tolerance mechanism of cadmium in Ceratopteris pteridoides: Translocation and subcellular distribution

• Published in: Ecotoxicology and environmental safety 2020-07, Vol.197, p.110599, Article 110599
• Main Authors: Bora, Monashree Sarma, Gogoi, Nirmali, Sarma, Kali Prasad
• Format: Article
• Language: English
• Subjects: Cadmium; Ceratopteris pteridoides; Detoxification mechanism; Oxidative stress; Subcellular distribution
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.110599

Hydroponic experiment was conducted to investigate the biochemical responses and accumulation behaviour of cadmium (Cd) in aquatic fern, Ceratopteris pteridoides, under four different levels of exposure. Plants were grown in 10 μM (CdT1), 20 μM (CdT2), 40 μM (CdT3) and 60 μM (CdT4) concentrations of Cd for 12 consecutive days and Cd accumulation in different plant parts, cell levels and growth medium was estimated. In C. pteridoides, Cd removal kinetics was best described by pseudo-second-order kinetic model. Increased accumulation of Cd in the plants was detected in a concentration dependent manner with maximum under 60 μM of Cd (CdT4) exposure (191.38 mg kg−1, 186.19 mg kg−1 and 1316.34 mg kg−1 in leaves, stems and roots, respectively). Cell wall of C. pteridoides is identified as crucial Cd storage site with the highest (28–69%) accumulation followed by organelles (14–44%) and soluble fraction (6–46%). Increased leaf proline, malondialdehyde (MDA) and protein content with significant reduction (P 1) bioconcentration factor (BCF) and lower ( leaf > stem.•Cell wall was the prime reservoir of Cd in Ceratopteris pteridoides under Cd exposure.•Ceratopteris pteridoides is found to be suitable for use in phytostabilization than phytoextraction.