A network model shows the importance of coupled processes in the microbial N cycle in the Cape Fear River Estuary

• Published in: Estuarine, coastal and shelf science coastal and shelf science, 2012-06, Vol.106, p.45-57
• Main Authors: Hines, David E., Lisa, Jessica A., Song, Bongkeun, Tobias, Craig R., Borrett, Stuart R.
• Format: Article
• Language: English
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; biogeochemistry; Biological and medical sciences; Brackish water ecosystems; coupling; ecological network analysis; Fundamental and applied biological sciences. Psychology; General aspects; microorganisms; nitrogen cycle; sediment Chemistry; Synecology
• ISSN: 0272-7714; 1096-0015
• DOI: 10.1016/j.ecss.2012.04.018

Estuaries serve important ecological and economic functions including habitat provision and the removal of nutrients. Eutrophication can overwhelm the nutrient removal capacity of estuaries and poses a widely recognized threat to the health and function of these ecosystems. Denitrification and anaerobic ammonium oxidation (anammox) are microbial processes responsible for the removal of fixed nitrogen and diminish the effects of eutrophication. Both of these microbial removal processes can be influenced by direct inputs of dissolved inorganic nitrogen substrates or supported by microbial interactions with other nitrogen transforming pathways such as nitrification and dissimilatory nitrate reduction to ammonium (DNRA). The coupling of nitrogen removal pathways to other transformation pathways facilitates the removal of some forms of inorganic nitrogen; however, differentiating between direct and coupled nitrogen removal is difficult. Network modeling provides a tool to examine interactions among microbial nitrogen cycling processes and to determine the within-system history of nitrogen involved in denitrification and anammox. To examine the coupling of nitrogen cycling processes, we built a nitrogen budget mass balance network model in two adjacent 1 cm3 sections of bottom water and sediment in the oligohaline portion of the Cape Fear River Estuary, NC, USA. Pathway, flow, and environ ecological network analyses were conducted to characterize the organization of nitrogen flow in the estuary and to estimate the coupling of nitrification to denitrification and of nitrification and DNRA to anammox. Centrality analysis indicated NH4+ is the most important form of nitrogen involved in removal processes. The model analysis further suggested that direct denitrification and coupled nitrification-denitrification had similar contributions to nitrogen removal while direct anammox was dominant to coupled forms of anammox. Finally, results also indicated that partial nitrification-anammox may play an important role in anammox nitrogen removal in the Cape Fear River Estuary. [Display omitted] ► We constructed a N mass balance network model for the Cape Fear River Estuary. ► Ammonium plays the most central role in N cycling in the Cape Fear River Estuary. ► We estimated the strength of coupling of N cycling processes using environ analysis. ► Coupled nitrification-denitrification comprises nearly half of denitrification loss. ► Coupled nitrification-anammox may be an impor