Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon

• Published in: Egyptian Journal of Basic and Applied Sciences 2018-03, Vol.5 (1), p.80-86
• Main Authors: Ngoua-Meye-Misso, Rick-Leonid, Sima-Obiang, Cédric, Ndong, Jean De La Croix, Ondo, Joseph Privat, Ovono Abessolo, Felix, Obame-Engonga, Louis-Clément
• Format: Article
• Language: English
• Subjects: Anti-inflammatory activity; Antiangiogenic activity; Antioxidant activity; Cancer; Lophira procera; Phytochemical screening
• ISSN: 2314-808X; 2314-808X
• DOI: 10.1016/j.ejbas.2017.11.003

•Extract of Lophira procera have a high content of phenolic compounds.•Extracts of Lophira procera exhibit strong antioxidant activity.•Good anti-inflammatory activity of Lophira procera.•Good antiangiogenic activity of water extract. The objective of this study was to perform phytochemical screening, to determine the content of phenolic compound, to evaluate antioxidant, anti-inflammatory and antiangiogenic activities of ethanol, water-ethanol and water extracts of Lophira procera. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phosphomolybdenum assay, anti-inflammatory activity by proteins denaturation inhibition and membranes stabilization test and antiangiogenic activity by chicken chorioallantoic membrane (CAM) method. The results showed that this plant is rich in saponins, polyphenols, tannins, total flavonoids, proanthocyanidins and coumarins. Extracts presented a strong antioxidant activity (IC50 values of 5.452 ± 0.119 μg/mL and 6.346 ± 0.544 μg/mL and respective AAI of 9.173 ± 0.203 and 7.919 ± 0.711). Excellent anti-inflammatory activity was also observed (IC50 = 16.952 ± 1.897 and IC50 = 23.172 ± 0.066 μg/mL for inhibition of protein denaturation and membrane stabilization respectively). Finally, extracts manifested a very good anti-angiogenic activity (with inhibitions ranging from 57.142 ± 0.124% to 100%). These biological activities are certainly due to high content of phenolic compound. This is the first study to report the phytochemical screening, the content of phenolic compound, the antioxidant, anti-inflammatory and antiangiogenic activities of extract derived from Lophira procera. The use of this plant in traditional medicine against ulcers, breast cancer, kidney and dental pain is therefore justified and its potential as a candidate for bioactive therapeutic molecule.