Cytotoxic and genotoxic effects of [Ru(phi) 3] 2+ evaluated by Ames/ Salmonella and MTT methods

In this work, we synthesized and evaluated the cytotoxic effect of [Ru(phi) 3] 2+, on rat C6 glioma cell line. Cell viability was determined by assay with 3-(4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mutagenicity of [Ru(phi) 3] 2+ was studied in vitro by using two strains...

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Bibliographic Details
Published in:European journal of medicinal chemistry 2009-06, Vol.44 (6), p.2601-2605
Main Authors: Benkli, Kadriye, Tunali, Yağmur, Cantürk, Zerrin, Artagan, Öge, Alanyali, Filiz
Format: Article
Language:English
Subjects:
9,10-Phenanthrenequinone diimine
Animals
Biological and medical sciences
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Chemical mutagenesis
Cytotoxic effect
DNA Repair - drug effects
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Frameshift Mutation
Genotoxicity
Glioma - drug therapy
Glioma - pathology
Medical sciences
Molecular Structure
Mutagenicity
Mutagenicity Tests
Organometallic Compounds - chemical synthesis
Organometallic Compounds - chemistry
Organometallic Compounds - pharmacology
Rats
Ru(II) complex
Salmonella typhimurium - drug effects
Salmonella typhimurium - genetics
Tetrazolium Salts - chemistry
Thiazoles - chemistry
Toxicology
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Summary:In this work, we synthesized and evaluated the cytotoxic effect of [Ru(phi) 3] 2+, on rat C6 glioma cell line. Cell viability was determined by assay with 3-(4-5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mutagenicity of [Ru(phi) 3] 2+ was studied in vitro by using two strains of Salmonella typhimurium with frameshift mutation (TA98) and base-pair substitution mutation (TA100) were used in plate incorporation assay in the absence of metabolic activation. According to the results, the Ru compound is not toxic but mutagenic, and it shows cytotoxic effect towards C6 rat glioma cells in100 μM. [Display omitted]
ISSN:0223-5234
1768-3254
DOI:10.1016/j.ejmech.2008.09.017