Puerarin protects rat pancreatic islets from damage by hydrogen peroxide

The protective effect of puerarin, an isoflavone purified from Chinese herb radix of Pueraria lobata, on hydrogen peroxide (H 2O 2)-induced rat pancreatic islets damage was investigated. Exposure of islets to 500 μM H 2O 2 could cause a significant viability loss and an increase in apoptotic rate. P...

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Bibliographic Details
Published in:European journal of pharmacology 2006-01, Vol.529 (1), p.1-7
Main Authors: Fu-Liang, Xiong, Xiao-Hui, Sun, Lu, Gan, Xiang-Liang, Yang, Hui-Bi, Xu
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Biological and medical sciences
Catalase - metabolism
Drugs, Chinese Herbal - pharmacology
Flow Cytometry
Hydrogen Peroxide - toxicity
In Vitro Techniques
Insulin
Insulin - metabolism
Insulin Secretion
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Islets of Langerhans - pathology
Isoflavones - pharmacology
Male
Medical sciences
Oxidants - toxicity
Oxidative stress
Pancreatic islet
Pharmacology. Drug treatments
Protective Agents - pharmacology
Puerarin
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
Superoxide Dismutase - metabolism
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Summary:The protective effect of puerarin, an isoflavone purified from Chinese herb radix of Pueraria lobata, on hydrogen peroxide (H 2O 2)-induced rat pancreatic islets damage was investigated. Exposure of islets to 500 μM H 2O 2 could cause a significant viability loss and an increase in apoptotic rate. Pretreatment of islets with puerarin for 48 h resulted in a reduction in viability loss and apoptotic rate. 100 μM puerarin significantly inhibited the apoptosis of islets induced by H 2O 2. In addition, preincubation with puerarin could restore the H 2O 2-induced decrease in basal and glucose-stimulated insulin secretion in pancreatic islets. Puerarin was also found to inhibit the free radicals production induced by H 2O 2 and to increase catalase and superoxide dismutase (SOD) activities in the isolated pancreatic islets. These results suggest that puerarin can protect islets against oxidative stress probably due to stimulating the activities of the antioxidant enzymes. Puerarin may be effective in preventing islet cells from the toxic action of reactive oxygen species in diabetes.
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2005.10.024