Blocking Interleukin-1 Beta Reduces the Evolution of Thoracic Aortic Dissection in a Rodent Model

• Published in: European journal of vascular and endovascular surgery 2020-12, Vol.60 (6), p.916-924
• Main Authors: Guo, Ling-Ling, Wu, Meng-Tao, Zhang, Li-Wei, Chu, Yong-Xin, Tian, Peng, Jing, Zai-Ping, Li, Jia-Si, Sun, Yu-Dong, Yeung, Kak K, Zhang, Lei
• Format: Article
• Language: English
• Subjects: Aminopropionitrile; Aneurysm, Dissecting - chemically induced; Aneurysm, Dissecting - metabolism; Aneurysm, Dissecting - pathology; Animals; Antibodies - pharmacology; Antibody; Aorta, Thoracic - pathology; Aortic Aneurysm, Thoracic - chemically induced; Aortic Aneurysm, Thoracic - metabolism; Aortic Aneurysm, Thoracic - pathology; Apoptosis; Biomechanics; Disease Models, Animal; Elastin - metabolism; Interleukin-1 beta; Interleukin-1beta - antagonists & inhibitors; Interleukin-1beta - immunology; Interleukin-1beta - metabolism; Male; Matrix metalloproteinase; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Rats; Rats, Sprague-Dawley; Surgery; Survival Rate; Thoracic aortic dissection
• ISSN: 1078-5884; 1532-2165
• DOI: 10.1016/j.ejvs.2020.08.032

ObjectiveThoracic aortic dissection (TAD) is associated with matrix changes, biochemical changes, and inflammatory markers like interleukin-1 beta (IL-1β). However, the exact mechanism remains unknown. This study aimed to investigate the role of IL-1β, matrix metalloproteinase (MMP)-2, MMP-9, smooth muscle cell apoptosis, and elastic fibre fracture in the development of TAD in a rat model. MethodsThe TAD rat model was induced by β-aminopropionitrile (BAPN). TAD was investigated in 112 male Sprague–Dawley rats, which were equally divided into four groups of 28 rats (Control, BAPN, BAPN + IL-1β, and BAPN + IL-1β antibody). Systolic blood pressure, survival, and the development of TAD were measured after six weeks. Expression of IL-1β, MMP-2, and MMP-9 was measured by Western blot. Apoptosis, aortic elastin concentration, and biomechanical characteristics were measured by the TdT mediated dUTP nick end labelling assay, Victoria blue staining, and in vitro testing. ResultsDuring six weeks, the mortality was 0% (0/28) in the control group, 53.6% (15/28) in the BAPN group ( p