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Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species
An electrochemical biosensor for specific detection of DNA sequences from Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the Aspergillus gene aflR that codes a biochemical pathway of aflatox...
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Published in: | Electrochemistry communications 2010, Vol.12 (1), p.106-109 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | An electrochemical biosensor for specific detection of DNA sequences from
Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the
Aspergillus gene
aflR that codes a biochemical pathway of aflatoxin B1 production.
Voltammetric detection of the specific
Aspergillus DNA sequence is based on hybridization of adsorbed target DNA with a biotinylated probe and subsequent binding with streptavidin alkaline phosphatase conjugate. Such modified electrode surface of carbon paste electrode (CPE) is incubated in a buffer with an electrochemically inactive substrate (1-naphthyl phosphate). Alkaline phosphatase converts 1-naphthyl phosphate into 1-naphthol, which is determined by the selected voltammetric technique. The optimized procedure provided good differentiation between complementary and noncomplementary probes and also between potentially aflatoxigenic and non-aflatoxigenic fungi of the genus
Aspergillus. |
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ISSN: | 1388-2481 1873-1902 |
DOI: | 10.1016/j.elecom.2009.10.047 |