An ultrasensitive electrochemical immunosensor for the detection of salbutamol based on Pd@SBA-15 and ionic liquid

A sandwich-type electrochemical immunosensor for the ultrasensitive detection of the salbutamol (SAL) antigen based on sodium dodecylbenzene sulfonate-functionalized graphene sheets (SDBS-GS), Pd nanoparticles in functionalized SBA-15 (Pd@SBA-15) and ionic liquid (IL) is described. The primary SAL a...

Full description

Saved in:
Bibliographic Details
Published in:Electrochimica acta 2012-05, Vol.69, p.79-85
Main Authors: Cui, Zhentao, Cai, Yanyan, Wu, Dan, Yu, Haiqin, Li, Yan, Mao, Kexia, Wang, Huan, Fan, Haixia, Wei, Qin, Du, Bin
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
General pharmacology
Graphene sheets
Ionic liquid
Medical sciences
Methods. Procedures. Technologies
Pd@SBA-15
Pharmacology. Drug treatments
Salbutamol antigen
Sandwich-type immunosensor
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A sandwich-type electrochemical immunosensor for the ultrasensitive detection of the salbutamol (SAL) antigen based on sodium dodecylbenzene sulfonate-functionalized graphene sheets (SDBS-GS), Pd nanoparticles in functionalized SBA-15 (Pd@SBA-15) and ionic liquid (IL) is described. The primary SAL antibody (Ab1) can be covalently attached to the sulfonic groups of SDBS-GS through the amines of Ab1 with an acyl chloride cross-linking reaction. Pd@SBA-15 was prepared by adsorption of H2PdCl4 onto the SBA-15 surface; the adsorbed H2PdCl4 was then reduced to Pd nanoparticles with sodium borohydride. Pd@SBA-15 was conjugated to the secondary SAL antibody (Ab2) through glutaraldehyde. IL was added to the mixture of Pd@SBA-15 and Ab2 to promote electron transport. The synergistic effect between IL and Pd@SBA-15 retained the bioactivity of Ab2. The sensitivity of the sandwich-type immunosensor using Pd@SBA-15/Ab2/IL for the detection of SAL was much higher than those that used either SBA-15/Ab2 or Pd@SBA-15/Ab2. Under optimal conditions, the electrochemical immunosensor exhibited a wide working range from 0.02 to 15.0ng/mL with a detection limit of 7pg/mL. The precision, reproducibility and stability of the immunosensor were acceptable. The proposed strategy could be easily extended to fabricate immunosensors for other tumor markers.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2012.02.073