Application of Centri-voltammetry to Cytosensors: Cyto-centrivoltammetry

In this work, a centri-voltammetric cytosensor suitable for the for detection of folate receptor positive cervical cancer (HeLa) cells was developed for the first time. In order to see the effect of centrifugation, developed cytosensor’s performance was compared in terms of analytical characteristic...

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Bibliographic Details
Published in:Electrochimica acta 2016-09, Vol.211, p.71-76
Main Authors: Tepeli, Yudum, Barlas, Fırat Barış, Timur, Suna, Anik, Ulku
Format: Article
Language:English
Subjects:
Centri-voltammetry
Electrochemical cytosensor
HeLa cells
PAMAM
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Summary:In this work, a centri-voltammetric cytosensor suitable for the for detection of folate receptor positive cervical cancer (HeLa) cells was developed for the first time. In order to see the effect of centrifugation, developed cytosensor’s performance was compared in terms of analytical characteristics values, in the presence and absence of centri-voltammetry. As a result, in the absence of centri-voltammetry, the linear range was found between 75cells/mL and 106 cells/mL with limit of detection (LOD) value as 75 cells/mL and with R.S.D value of 3.5% (for 5.0×104 HeLa cells/mL (n=3)). Meanwhile in the presence of centri-voltammetry, the linear range was found between 8.0 cells/mL and 5×106 cells/mL and LOD value was calculated as 8 cells/mL with R.S.D value of 4.7% for the same concentration.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2016.06.030