Loading…

Bio-inspired graphene nanocomposite enabled electrochemical immunosensor for detection and quantification of NS1 protein of dengue virus

Dengue is a serious illness that affects a large portion of the global population. Infection with the dengue virus may have been silent or symptomatic, making identification crucial for tracking the virus's propagation and protecting society from its catastrophic consequences. The non-structura...

Full description

Saved in:
Bibliographic Details
Published in:Electrochimica acta 2024-01, Vol.475, p.143630, Article 143630
Main Authors: Chaturvedi, Mansi, Patel, Monika, Mondal, D.P., Srivastava, Avanish Kumar, Dwivedi, Neeraj, Dhand, Chetna
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dengue is a serious illness that affects a large portion of the global population. Infection with the dengue virus may have been silent or symptomatic, making identification crucial for tracking the virus's propagation and protecting society from its catastrophic consequences. The non-structural dengue protein NS1 has been used as a biomarker in the diagnosis of dengue because it circulates widely in the blood during the acute phase of the infection and is associated with viremia. In this study, we have reported the development of an electrochemical immunosensor using nanobio-inspired platform composed of ternary nanocomposite of reduced graphene oxide (rGO), polydopamine (pDA) and gold nanoparticles (AuNPs) immobilized with Anti-NS1 antibodies for the detection of NS1 protein. The nanocomposite formation and antibody immobilization are confirmed using various microscopic, spectroscopic and electrochemical techniques. The differential pulse voltammetry (DPV) has been used to investigate the detailed electrochemical properties and immunosensing response of the developed immunosensor device. The designed immunosensor showed linear detection response for NS1 protein from 1 ng ml−1 to 100 µg ml−1 with a sensitivity of 1.78 ng ml−1 mA−1 and good shelf life. This developed immunosensor with exceptionally low detection limit revealed its potential for early diagnosis of dengue virus infection.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2023.143630