Electropermeabilization responses in Gram-positive and Gram-negative bacteria

Effects of 3-kJ kg−1 nanosecond pulsed electric fields (PEFs) on cellular permeabilization of Salmonella enterica and Staphylococcus aureus were observed. It was seen that bacterial responses depend on both the electrical pulse attribute and the cell plasma membrane structure. For traditional permea...

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Bibliographic Details
Published in:Journal of electrostatics 2013-08, Vol.71 (4), p.773-777
Main Authors: Chaturongakul, Soraya, Kirawanich, Phumin
Format: Article
Language:English
Subjects:
Bacterial inactivation
Cell wall structure
Electropermeabilization
Pulsed electric field
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Summary:Effects of 3-kJ kg−1 nanosecond pulsed electric fields (PEFs) on cellular permeabilization of Salmonella enterica and Staphylococcus aureus were observed. It was seen that bacterial responses depend on both the electrical pulse attribute and the cell plasma membrane structure. For traditional permeabilization, the responses involved the thickness of the peptidoglycan layer where a maximum of 2.5 log reduction in S. enterica population was achieved. Meanwhile, in the area of selective permeabilization, it showed insignificant reduction in both pathogens. Such inactivation mechanisms were described through the behavior of potential across plasma membrane and intracellular organelles by PSPICE simulations incorporating PEF-cell interaction model. •We examine Salmonella enterica and Staphylococcus aureus in activation responses to PEF exposure.•Responses depend on the area of electrical permeabilization and membrane structure.•Bacterial inactivation was described through the behavior of cellular voltages.•Studies were done with PSPICE simulations incorporating PEF-cell interaction.
ISSN:0304-3886
1873-5738
DOI:10.1016/j.elstat.2013.06.005