Responses of ectomycorrhizal Populus tremuloides and Betula papyrifera seedlings to salinity

• Published in: Environmental and experimental botany 2008-04, Vol.62 (3), p.357-363
• Main Authors: Yi, Huang, Calvo Polanco, Mónica, MacKinnon, Michael D., Zwiazek, Janusz J.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Betula papyrifera; Biological and medical sciences; chlorides; dry matter accumulation; Economic plant physiology; Ectomycorrhizae; forest trees; Fundamental and applied biological sciences. Psychology; Hebeloma crustuliniforme; ion transport; Laccaria bicolor; leaves; mycorrhizal fungi; plant damage; Populus tremuloides; Root hydraulic conductance; root hydraulic conductivity; roots; Salt exclusion; salt stress; Salt tolerance; seedlings; shoots; sodium; sodium chloride; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); temperate forests; Transpiration
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2007.10.008

Roots of trembling aspen ( Populus tremuloides Michx.) and paper birch ( Betula papyrifera Marsh.) seedlings were inoculated with Hebeloma crustuliniforme or Laccaria bicolor and treated with 25 mM NaCl for 6 weeks. Both tree species appeared to be relatively tolerant of the applied NaCl treatment and did not develop visible leaf symptoms that are characteristic of salt injury. Salt treatment reduced total dry weights in aspen and birch, but did not significantly affect transpiration rates and root hydraulic conductance. Salt-treated ectomycorrhizal aspen maintained higher root hydraulic conductance compared with non-mycorrhizal plants. Na and Cl concentrations increased in shoots and roots of mycorrhizal and non-mycorrhizal aspen and birch in response to NaCl treatment. Roots of NaCl-treated aspen inoculated with H. crustuliniforme had over twofold higher concentrations of Na compared with non-mycorrhizal NaCl-treated plants. Similarly to aspen, Na and Cl concentrations increased in roots and shoots of NaCl-treated birch seedlings. However, in birch, there were no significant differences in Na and Cl concentrations between mycorrhizal and non-mycorrhizal plants. The results suggest that salt exclusion by the ectomycorrhizal associations is host-specific or/and that the processes leading to salt exclusion are activated in ectomycorrhizal plants by a threshold salt level which may vary between plant species.