Ketocarotenoids accumulation in the leaves of engineered Brassica napus restricts photosynthetic efficiency and plant growth

•Astaxanthin successfully synthesized in Brassica napus leaves.•Endogenous chlorophyll and carotenoids content decreased in transgenic plants.•NPQ decreased and PSII was impaired in transgenic plants.•Photosynthetic capacity and plant growth were negatively affected. Astaxanthin is a high-valued ket...

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Bibliographic Details
Published in:Environmental and experimental botany 2021-06, Vol.186, p.104461, Article 104461
Main Authors: Wang, Jie-Lin, Tan, Shun-Ling, He, Ming-Xia, Huang, Wei, Huang, Jun-Chao
Format: Article
Language:English
Subjects:
Astaxanthin
Brassica napus
Ketocarotenoids
Photoprotection
Photosystem II (PSII)
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Summary:•Astaxanthin successfully synthesized in Brassica napus leaves.•Endogenous chlorophyll and carotenoids content decreased in transgenic plants.•NPQ decreased and PSII was impaired in transgenic plants.•Photosynthetic capacity and plant growth were negatively affected. Astaxanthin is a high-valued ketocarotenoid that is rarely synthesized in higher plants due to their lack of a carotenoid ketolase. Overexpression of a heterologous β-carotene ketolase gene coupled with other pathway genes in higher plants has triggered the production of various ketocarotenoids in the transformants. Here we reported that constitutive and simultaneous overexpression of four genes involved in astaxanthin biosynthesis in Brassica napus resulted in the accumulation of the nonnative ketocarotenoids such as astaxanthin and ketolutein, and the decreased production of chlorophylls, lutein and violaxanthin in leaves. As a result, transgenic plants showed lower non-photochemical quenching (NPQ) under high light, leading to moderate photoinhibition of photosystem II (PSII). Such PSII photoinhibition depressed linear electron transport and thus restricted the rate of CO2 assimilation. Consequently, transgenic plants grew slower and showed less biomass than wild type. These results indicated that endogenous synthesis of ketocarotenoids negatively affected photosynthesis and impaired plant growth in B. napus. Therefore, to prevent the side effect on normal growth, seed specific expression of the transgenes may be necessary for enhanced production of astaxanthin in seeds.
ISSN:0098-8472
1873-7307
DOI:10.1016/j.envexpbot.2021.104461