Mining natural genetic variations for nitrogen use efficiency utilizing nested synthetic hexaploid wheat introgression libraries

• Published in: Environmental and experimental botany 2023-08, Vol.212, p.105394, Article 105394
• Main Authors: Sandhu, Nitika, Sethi, Mehak, Kaur, Harpreet, Dhillon, Amandeep, Kumar, Aman, Kaur, Amandeep, Kaur, Satinder, Varinderpal-Singh, Bentley, Alison R., Chhuneja, Parveen
• Format: Article
• Language: English
• Subjects: Genome wide association studies; Marker-trait association; Nitrogen use efficiency; Wheat
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2023.105394

Wheat is grown on more than 240 million hectares globally, more than any other commercial crop. Variation for nitrogen-use efficiency (NUE) in wheat germplasm is very important as efficiency of nitrogen fertilizers use can have significant effect on overall consumption of N. In the present study we used nested synthetic hexaploid wheat introgression libraries to detect genetic variation associated with NUE and related traits. The libraries were genotyped with 9474 SNP markers and used for genome-wide association mapping. Significant phenotypic variation was observed for all measured and derived traits. We detected 10 Quantitative trait locus (QTL) and 19 marker-trait associations (MTAs) possibly involved in improving NUE. Of these, 5 QTL and 8 MTAs detected under nitrogen-limited conditions have potential for use in breeding to increase nitrogen-deficiency tolerance. Nitrate transporter genes collocated with detected MTAs showed significant changes in expression of TaNRT2 genes in response to N-starvation and N-recovery. The identified promising breeding lines with stable yield, better NUE and acceptable protein content may constitute an important genetic resource in improving NUE of modern wheat varieties. •To meet future wheat production demands, improving nitrogen use while maintaining grain yield is vital.•We identified marker-trait associations improving nitrogen use efficiency while maintaining grain yield under varying nitrogen levels.•We also identified promising breeding lines with significant genetic variations and carrying the trait-associated markers or candidate genes.•These may serve as potential donors to be exploited further in genomics-assisted breeding programs targeting improved NUE while maintaining grain yield in wheat.