Higher endogenous abscisic acid confers greater tolerance to saline-alkaline stress in Petunia hybrida

• Published in: Environmental and experimental botany 2024-12, Vol.228, p.106035, Article 106035
• Main Authors: Liu, Xueqi, Niu, Hongzhuang, Li, Jiazhe, Jiang, Decheng, Chen, Ruihong, Zhang, Rui, Li, Qian
• Format: Article
• Language: English
• Subjects: ABA; Na+/K+ homeostasis; Osmotic regulation; Petunia hybrida; ROS detoxifying; Saline-alkaline stress
• ISSN: 0098-8472
• DOI: 10.1016/j.envexpbot.2024.106035

Saline-alkaline stress not only leads to reduced crop yields but also diminishes the ornamental value of flowers. While petunia exhibits tolerance to saline-alkaline stress, research on the mechanisms underlying this tolerance remains unreported. We investigated the physiological and molecular mechanisms underlying saline-alkaline stress tolerance using two petunia genotypes (Haishishenlou and Mitchell Diploid) with differing tolerance levels. Haishishenlou exhibited less inhibition of growth under saline-alkaline stress compared to Mitchell Diploid, as indicated by higher biomass. Higher endogenous concentration of abscisic acid (ABA) and greater expression levels of ABA biosynthetic genes and lower expression levels of ABA catabolic genes in Haishishenlou than in Mitchell Diploid were observed when challenged by saline-alkaline stress, suggesting that a higher concentration of ABA may underpin the greater tolerance of Haishishenlou to saline-alkaline stress than that of Mitchell Diploid. Under saline-alkaline conditions, Haishishenlou displayed higher chlorophyll content, photosynthetic rates, Pro and soluble sugars content, as well as higher activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD), and a lower Na+/K+ ratio. Exogenous application of ABA alleviated the growth inhibition induced by saline-alkaline stress, promoted the accumulation of proline and soluble sugar, reduced reactive oxygen species (ROS) content and Na+/K+ ratio, and improved antioxidative capacity. These results indicated that a higher endogenous concentration of ABA may underpin the greater tolerance of petunia seedlings to saline-alkaline stress, and exogenous ABA improves the saline-alkaline tolerance of petunia seedlings. •Growth of Haishishenlou was less inhibited by saline-alkaline compared to Mitchell due to higher endogenous abscisic acid.•Exogenous application of abscisic acid alleviates saline-alkaline-induced inhibition by reducing the Na+/K+ ratio.•Exogenous application of abscisic acid alleviates saline-alkaline-induced inhibition by enhancing osmotic regulation.•Exogenous application of abscisic acid alleviates saline-alkaline-induced inhibition by improving antioxidative capacity.