p53-dependent apoptosis contributes to di-(2-ethylhexyl) phthalate-induced hepatotoxicity

• Published in: Environmental pollution (1987) 2016-01, Vol.208 (Pt B), p.416-425
• Main Authors: Ha, Mei, Wei, Li, Guan, Xie, Li, Lianbing, Liu, Changjiang
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Cell Line; DEHP; Diethylhexyl Phthalate - toxicity; DNA Damage - drug effects; Hepatotoxicity; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Oxidative Stress - drug effects; p53; Proliferating Cell Nuclear Antigen - genetics; Proliferating Cell Nuclear Antigen - metabolism; Proto-Oncogene Proteins c-mdm2 - genetics; Proto-Oncogene Proteins c-mdm2 - metabolism; Rats, Sprague-Dawley; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2015.10.009

Di-(2-ethylhexyl) phthalate (DEHP) is used extensively in many personal care and consumer products, resulting in widespread non-occupational human exposure through multiple routes and media. DEHP has various deleterious effects including hepatotoxicity. p53 protein is a central sensor in cell apoptosis. In order to clarify the roles of p53 in DEHP-induced hepatotoxicity, Sprague–Dawley (SD) rats were dosed daily with DEHP by gavage for 30 days; BRL cells (rat liver cell line) were treated with DEHP for 24 h after pretreatment with NAC or small interfering RNA (siRNA). Results indicated that after exposure to DEHP, hepatic histological changes such as hepatocyte edema, vacuolation and hepatic sinusoidal dilation, and increased apoptosis index were observed. In the liver, DEHP induced oxidative stress and DNA damage, which activated p53 in vivo and in vitro. Pretreatment with NAC significantly reduced ROS level and p53 expression in BRL cells. The suppressed Mdm2 also contributed to p53 accumulation. Activated p53 mediated hepatocyte apoptosis via the intrinsic mitochondrial pathway, inhibiting anti-apoptotic Bcl-2 and Bcl-xL and inducing pro-apoptotic Bax, cytochrome c and caspases. In p53-silenced BRL cells, hepatocyte apoptosis mediated by p53 was attenuated. PCNA protein level was upregulated after p53 gene silencing. However, the Fas/FasL apoptotic pathway did not exhibit activated signs in DEHP-caused hepatotoxicity. Taken together, DEHP-caused oxidative stress and Mdm2 downregulation contribute to p53 activation. The p53-dependent apoptotic pathway plays critical and indispensable roles in DEHP-induced hepatotoxicity, while the Fas/FasL pathway does not involve in this molecular event. •DEHP induced oxidative stress and DNA damage.•Oxidative stress and Mdm2 suppression contributed to p53 activation.•Activated p53 mediated hepatocyte apoptosis via the mitochondrial pathway.•DEHP led to hepatic histological changes via p53-dependent apoptosis.•The Fas/FasL apoptotic pathway did not involve in DEHP-caused hepatotoxicity. The p53-dependent apoptotic pathway plays critical and indispensable roles in DEHP-induced hepatotoxicity.