The toxicity of 2,6-dichlorobenzoquinone on the early life stage of zebrafish: A survey on the endpoints at developmental toxicity, oxidative stress, genotoxicity and cytotoxicity

• Published in: Environmental pollution (1987) 2019-02, Vol.245, p.719-724
• Main Authors: Sun, Hong-Jie, Zhang, Yu, Zhang, Jing-Ying, Lin, Hongjun, Chen, Jianrong, Hong, Huachang
• Format: Article
• Language: English
• Subjects: Cytotoxicity; Dichlorobenzoquinone; Genotoxicity; Lipid peroxidation; Oxidative stress; Zebrafish
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2018.11.051

2,6-dichlorobenzoquinone (2,6-DCBQ), an emerging disinfection by-production, frequently occurs in reclaimed water and drinking water. However, limited information was available regarding its toxicity. To evaluate its impact, zebrafish at early life stage were exposed to 0, 10, 30, 60, 90, or 120 μg L−1 2,6-BDCQ for 72 h. Our results indicated that 2,6-BDCQ decreased zebrafish's survival rate to 65% and 44% at 90 and 120 μg L−1 treatments and increased its aberration rate to 11% and 26% at 90 μg L−1 and 120 μg L−1 treatments. Besides, 2,6-BDCQ had adverse effect on its oxidative stress (elevated superoxide dismutase activity), lipid peroxidation (increased malondialdehyde levels), DNA damage (increased 8-hydroxydeoxyguanosine contents) and apoptosis (increased caspase-3 activity). Although lower concentrations (≤60 μg L−1) of 2,6-BDCQ didn't exhibit significant effect on its survival development or lipid peroxidation of zebrafish, they induced obvious DNA damage and apoptosis occurrence. These results revealed 2,6-BDCQ caused genotoxicity and cytotoxicity to zebrafish. This study provides novel insight into 2,6-DCBQ-induced toxicity in zebrafish. [Display omitted] •≥ 90 μg L−1 2, 6-BDCQ significantly decreased survival rate and increased aberration rate.•2,6-BDCQ significantly induced lipid peroxidation and disturbed fatty acid metabolism.•2,6-BDCQ exert powerful genotoxicity, which can significantly cause DNA damage and influence DNA repair capacity, even at 10 μg L−1 treatment.•2,6-BDCQ exert powerful cytotoxicity, which can obviously induce apoptosis, even at 10 μg L−1 treatment. 2,6-BDCQ caused genotoxicity and cytotoxicity to zebrafish at early life stage.